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Chemical Screening and Antibacterial Activity of Honey Produced in Benin


  • Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604 Cotonou, Benin
  • Laboratoire de Protection des Végétaux, de Pathologie et Parasitologie des Abeilles, Faculté d’Agronomie, Université de Parakou, Benin
  • Laboratoire de Biomembrane et de Signalisation Cellulaire. Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Benin
  • Unité de Recherche en Microbiologie Appliquée et Pharmacologie des substances naturelles (U.R.M.A.Pha), Laboratoire de Recherche en Biologie Appliquée (L.A.R.B.A), Ecole Polytechnique d’Abomey-Calavi (E.P.A.C), Université d’Abomey-Calavi (U.A.C), Benin


Objectives: This study aimed to evaluate the antibacterial activity of honeys produced in Benin against selected pathogenic strains and to determine their composition in plant secondary metabolites with known antibacterial activity. Methods: A total of 60 honey samples were collected in the country’s three phyto-geographical zones during the dry and rainy seasons. Chemical screening for secondary metabolites was performed on raw undiluted honey. Undiluted honey (100%) and honey diluted at 75%, 50% and 25% were used to assess their antimicrobial effect in vitro on six reference strains (ref-S) and six meat isolated staphylococcal strains (meat-S). MIC and MBC were also determined. Results: Chemical screening of undiluted honeys revealed that tannins, flavonoids, leuco-anthocyanins, alkaloids, coumarins and reducing compounds were preponderant irrespective of season of production. Only the undiluted honeys were effective at inhibiting some of the strains, namely four ref-S (P. mirabilis, S. aureus, S. epidermidis and S. oralis) and three meat-S (S. aureus, S.lentus, S. xylosus). Season of honey production had a significant effect on mean Inhibition Zone Diameter (IZD) for ref-S. For these strains, greater IZD were found for honeys produced during the dry season, except for S. aureus. For meat-S, in contrast, zone of production had a significant effect, with greater IZD for honeys from the Sudanian zone. Strains differed significantly in their sensitivity assessed by IZD: across all honeys, the greatest IZD were against P. mirabilis and meat-S:S. aureus and S. lentus. MIC varied greatly among honeys and strains. For meat-S, the effects of production zone and season were significant and the highest MIC were found for honeys from the Sudanian zone. Application/Improvements: Benin honey has bacteriostatic properties against several pathogens, and influenced by season and zone of production. Further studies are warranted to substantiate these new findings and identify the active principles.


Antimicrobial Activity, Benin, Honey, Human Pathogens, Phyto-Geographical Zone, Season of Production

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  • Anani K, Hudson JB, Souza C, Akpagana K, Tower GH, Arnason JT, Gbeassor M. Investigation of medicinal plants of Togo for antiviral and antimicrobial activities. Pharmaceutical Biology. 2000; 38(1):40–5. Crossref
  • Dabboussi F, El Omari K, Mouzawak M, Bayssari C, Hamze M. Recherche de salmonella listeria et de bactéries résistantes aux céphalosporines de troisième génération dans du fromage akkawi au nord du Liban. Lebanese Science Journal. 2013; 14(1):3–14.
  • Adejuwon AO, Agbaje EO, Idika N. Antifungal and antibacterial activities of aqueous and methanolic root extracts of Carica papayalinn. International Research Journal of Microbiology. 2011; 2:270–7.
  • Sina H, Baba-Moussa F, Ahoyo TA, Mousse W, Anagonou S, Gbenou JD, Prévost G, Kotchoni SO, Baba-Moussa L. Antibiotic susceptibility and Toxins production of Staphylococcus aureus isolated from clinical samples from Benin. African Journal Microbiology Research. 2011; 5(18):2797–803. Crossref
  • Akoua-Koffi C, Guessennd N, Gbonon V, Faye-Kette AYH, Dosso M. Methicillin resistant of staphylococcus aureus in Abidjan (1998-2001): A new hospital problem. Médecines et Maladies Infectieuses. 2004; 34(3):132–6. Crossref PMid:15617354
  • Akinyemi KO, Oladapo O, Okwara C E, Ibe CC, Fasure KA.Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for antimethicillin resistant Staphylococcus aureus activity. BMC Complementary and Alternative Medicine. 2005; 5:6–12. Crossref PMid:15762997 PMCid:PMC1079793
  • Liu JR, Ye YL, Lin TY, Wang YW, Peng CC. Effect of floral sources on the antioxidant antimicrobial and anti-inflammatory activities of honeys in Taiwan. Food Chemistry. 2013; 139(1-4):146–74. Crossref PMid:23561193
  • Baltrušaitytė V, Venskutonis PR, Čeksterytė V. Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chemistry; 2007. p. 502–14. Crossref
  • Badawy OF, Shafii SS, Tharwat EE, Kamal AM. Antibacterial activity of bee honey and its therapeutic usefulness against Escherichia coli O157:H7 and Salmonella typhimurium infection. Revue Scientifiqueet Technique. 2004; 23(3):1011–22. Crossref
  • Rossant A, Desmouliere A. Le miel, uncomposécomplexe aux propriétéssurprenantes. Thèse de doctorat en Pharmacie, Université de Limoges: Limoges; 2011. p. 132.
  • Mandal MD, Mandal S. Honey: Its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine. 2011; 1(2):154–60. Crossref
  • Couquet Y, Desmoulière A, Rigal M. Les propriétés antibactériennes et cicatrisantes du miel. Actualités pharmaceutiques. 2013; 52(531):22–5. Crossref
  • Laallam H, Boughediri L, Bissati S, Hammoudi R. Activité bactériostatique des miels du Sud Algérien. Revue des Bio Ressources. 2014; 4(1). Crossref
  • Molan PC. The antibacterial activity of honey. Symposium Tel Aviv; 1997. p. 27–37.
  • Al-Waili N, Al Ghamdi A, Ansari MJ, Al-Attal Y, Al-Mubarak A, Salom K. Differences in composition of honey samples and their impact on the antimicrobial activities against drug multiresistant bacteria and pathogenic fungi. Archives of Medicinal Research. 2013; 44(4):307–16. Crossref
  • Sinsin B, EyogMatig O, Assogbadjo AE, Gaoué OG, Sinadouwirou T. Dendrometric characteristics as indicators of pressure of Afzeliaafricana Sm. trees dynamics in different climatic zones of Benin. Biodiversity and Conservation. 2004; 13:1555–70. Crossref
  • Attien P, Sina H, Moussaoui W, Dadié T, ChabiSika K, Djéni T, Bankole HS, Kotchoni SO, Edoh V, Prévost G, Djè M, Baba-Moussa L. Prevalence and antibiotic resistance of Staphylococcus strains isolated from meat products sold in Abidjan streets (Ivory Coast). African Journal of Microbiology Research. 2013; 7(26):3285–93. Crossref
  • Houghton P, Raman A. Laboratory Handbook for fractionation of natural extracts. Pharmacognosy Research Laboratories. Departement of Pharmacy; King’s College, London; 1998. p. 98. Crossref
  • Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 1966; 45:493–6. PMid:5325707
  • SFM Société Française de Microbiologie Recommandations du Comité de l’Antibiogramme de la Société Française de Microbiologie; 2008. p. 49.
  • Delarras C. Microbiologie. 90 heures de travaux pratiques. Gaétan Morien Editeur; 1998. p. 169–78.
  • Moroh JLA, Bahi C, Dje K, Loukou YG, Guede-guina F. Étude de l’activitéantibactérienne de l’extraitacétatique (EAC) de Morindamorindoides(Baker) milne-redheat (rubiaceae) sur la croissancein-vitro des souchesd’Escherichia coli. Bulletin de la Société Royale des Sciences de Liège. 2008; 77:44–61.
  • El Sohaimy SA, Masry SHD, Mohamed G, Rashad H. Evaluation of Functional Properties of Local and Imported Honey in Egypt. American-Eurasian Journal of Agricultural & Environmental Sciences. 2015; 15(6):1147–54.
  • Al-Naama RT. Evaluation of in-vitro inhibitory effect of honey on some microbial isolates. Journal of Bacteriology Reseach. 2009; 1(6):64–7.
  • Abd-El AAM, El-Hadidy MR, El-Mashad NB, El-Sebaie AH. Antimicrobial effect of bee honey in comparison to antibiotics on organisms isolated from infected burns. Annals of Burns Fire Disasters. 2007; 20(2):83–8.
  • Merah M, Bensac I, Bachagha M, Bouderhem A. Etude de l’effet antimicrobien de trois échantillons du miel naturel recoltes du territoire Algérien. Annales des Sciences et Technologie. 2010; 2(2):115–25.
  • Fangio MF, Iurlina MO, Fritz R. Antimicrobialactivity of honey the southeast of Buenos Aires Province against Escherichia coli. Revista Argentina de Microbiologia. 2007; 39(2):120–3. PMid:17702261
  • Moussa A, Noureddine D, Abdelmelek M, Saad A. Antibacterial activity of various honey types of Algeria against Pathogenic Gram-Negative Bacilli: Escherichia coli and Pseudomonas aeruginosa. Asian Pacific Journal of Tropical Disease; 2012. p. 211–4.
  • Akinnibosun FI, Itedjere E. Evaluation of the antibacterial properties and synergistic effect of Garcinia kola Heckel (Family: Guttiferae) seed extract and honey on some bacteria. African Journal of Microbiology Research. 2013; 7(3):174–80.
  • Aurongzeb M, Azim MK. Antimicrobial properties of natural honey: a review of literature. Pakistan Journal of Biochemical Molecular and Biology. 2011; 44(3):118–24.
  • Sherlock O, Dolan A, Athman R, Power A, Gethin G, Cowman S, Humphreys H. Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus Escherichia coli and Pseudomonas aeruginosa. BMC Complementary and Alternative Medicine. 2010; 10(47):1– 5. Crossref
  • Halawani E, Shohayeb M. Survey of the antibacterial activity of Saudi and some international honeys. Journal of Microbiology and Antimicrobiogy. 2011; 3(4):94–101.
  • Kuncic MK, Jaklic D, Lapanje A, Gunde-Cimerman N. Antibacterial and antimycotic activities of Slovenian honeys. British Journal of Biomedical Science. 2012; 69(4):154–8.
  • Sharma N, Negi S, Kumar A, Patil S, Kumar A. Comparative Antimicrobial Potential of Raw & Commercial Honey Against Various Bacteria Isolated From Wound and Throat Samples. Asian Journal of Biochemical and Pharmaceutical Research. 2012; 2(2):31–9.
  • Ahmadi FM, Hendi SS, Alikhani MY, Khamverdi Z. Antibacterial Activity of Honey on Cariogenic Bacteria. Journal of Dentistry of Tehran University of Medical Sciences. 2013; 10(1):10–5.
  • Hammoudi, Bouderhem. L’effet antimicrobien du miel, mémoire de fin d’études supérieures en Biologie Option Microbiologie Faculté des sciences de la Nature de la vie de la terre et de l’Univers. Universite Kasdi-Merbah Ouargla; 2009. p. 72.
  • Lusby PE, Coombes AL, Wilkinson JM. Bactericidal activity of different honeys against pathogenic bacteria. Archives of Medical Research. 2005; 36(5):464–7. Crossref PMid:16099322


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