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In vitro Studies on Bactericidal Activity and Sensitivity Pattern of Isolated Marine Microalgae against Selective Human Bacterial Pathogens
Ten microalgae cultured under controlled condition were tested for their antimicrobial activity against the selective bacterial pathogens in compliance with paper disk method. Five different solvents were used for the extraction. Isochrysis galbana showed overall inhibition of [16.22%] followed by Chlorella marina [14.43%], Nannochloropsis oculata [14.07%], Dunaliella salina [13.91%] and Pavlova lutheri [13.17%]. These five microalgal strains were further investigated to examine concentration dependent microbicidal activity using tube dilution method. Microalgal strains were also investigated with agar-well diffusion method to understand the efficacy of antimicrobial principles against various bacterial pathogens. The findings in this study reveal that optimal activity is maintained by butanol extracts on Gram-positive bacteria; ethanol and petroleum ether extracts on both Gram-positive and Gram-negative bacteria; methanol extracts on Gram-negative organisms. Chloroform extracts, on the other hand did not show any significant antimicrobial activity.
Bactericidal, Disk Diffusion, Microalgae, Antibiotics, Pathogens
- Baker JT and Murphy V (1981) Compounds from Marine Organisms. CRC Press, Florida.
- Caccamese S and Azzolina R (1979) Screening for antimicrobial activities in marine algae from Eastern Sicily. Planta med. 37, 333-339.
- Caccamese S, Azzolina R, Furnari G, Furnari M, Cormaci M and Grasso S (1981) Antimicrobial and antiviral activities of some marine algae from eastern Sicily. Bot. Mar. 24, 367.
- Chapman DJ (1980) Seaweeds and their uses. Chapman & Hall, London.
- Cooper S, Battat A, Marot P and Sylvester M (1983) Production of antibacterial activities by two bacillariophyceae grown in dialysis culture. Can. J. Microbiol. 29, 338-341.
- Desta B (2005) Antimicrobial activity of 63 medicinal plants. J. Ethnopharmacol. 100, 168-175.
- Farnsworth NR, Akerele O, Bingel AS, Soejarto DD and Guo Z (1985) Medicinal plants in therapy. Bull. WHO. 63, 965-981.
- Faulkner DJ (I986) Marine natural products. Natural Products Report. 3, 1-33.
- Findlay JA and Patil AD (1984) Antibacterial constituents of the diatom Navicula delognei. J. Natural Products. 47, 815-818.
- Gopinathan CP (1996) Life feed culturemicroalgae. Bull.Cent.Mar.Fish.Inst.48, 110-116.
- Harder R (1917) Z. Botan. 9, 145-242.
- Isacc SW and Hedge GR (1987) Freshwater algae of Karnataka state, Cartamman taxa from Uttara Kannada District: new flora of Kartae. Phykos. 26,123-129.
- Kellam SJ, Cannell RJP, Owsianka AM and Walker JM (1988) Results of a large scale screening programme to detect antifungal activity from marine and freshwater microalgae in laboratory culture. British Phycol. J. 23, 45-47.
- Kellar SJ and Walker JM (1989) Antibacterial activity from marine microalgae in laboratory culture. Br. Phycol. J. 24,191-194.
- Khan NH, Rahman M and Kamal Nur-E (1988) Antibacterial activity of Euphorbia thymifolia Linn. Indian J. Med. Res. 87, 395-397.
- Kim Se-Kwon, Jeon You-Jin, Kim Won-Suk, Back Ho-Cheol, Park Pyo-Jam, Byun Hee-Guk and Bai Sungchul C (2001) Biochemical Composition of Marine Microalgae and their potential Antimicrobial Activity. J. Fisheries Sci. & Technol. 4 (2), 75.
- Laing I (1991) Cultivation of marine unicellular algae. MAFF Laboratory Leaflet Number 67. Directorate of Fisheries Research Lowestoft, UK. pp: 31.
- Mayer AMS and Gustafson KR (2003) Marine pharmocology in 2000: Antitumour and cytotoxic compounds. Intl. J. Cancer. 109, 291-299.
- Miura Y, Sode K, Magasaki Y and Matsunga T (1993) Light induced antimicrobial activity of extracts from marine Chlorella. J. Mar. Biotechnol. 1, 143-146.
- Moreau JD, Pasando P, Bernand P, Caram B and Pinnat JC (1988) Seasonal variation in the production antifungal substrates by some dictyotales (brown algae) from the French Mediterranean coast. Hydrobiologia. 2, 1097-1132.
- Padmakumar K and Ayyakannu K (1986) Antimicrobial activity of some marine algae of Porto Novo and Pondichery waters, East coast of India. J. Mar. Sci. 13, 90-91.
- Padmini SR, Rao PS and Karmarkar SM (1986) Antibacterial substances from brown algae, efficiency of solvents in the evaluation of antibacterial substances from Sargassum johnstonii. Bot. Mar. 29, 503-507.
- Pesando D and Caram B (1984) Screening of marine algae from the French Mediterranean coast for antibacterial and antifungal activity. Bot. Mar. 27, 381-386.
- Rao PS (1991) Biological investigation of marine algae screening of some green, red, brown seaweeds for their antimicrobial activity. Seaweed Res. Utiln. 14, 37-43.
- Regini BGS (2004) Studies on the distribution and seasonal abundance of microflora in estuarine environment of Kanyakuamri district. Ph.D. Thesis, Manonmaninam Sundaranar Univ., Tirunelveli, Tamil Nadu, India. pp: 96-97.
- Reichelt JL and Borowitzk MA (1984) Antimicrobial activity from marine algae: Results of a large scale screening program. Hydrobiologia. 116/117, 158-170.
- Richard JP, Owsianka AM and Walker JM (1988) Results of large scale screening programme to detect antibacterial activity from freshwater algae. Br. Phycol. J. 2, 41-44.
- Sastry VM and Rao GRK (1994) Antibacterial substances from marine algae: successive extraction using benzene, chloroform and methanol. Bot. Marina. 37, 357-360.
- Siddhanta AK, Mody KH, Ramavat BK, Chauhan VD, Garg HS, Goel AK, Jinandra Doss M, Srivastava MN, Patnaik GK and Kamboj VP (1997) Bioactivity of marine organisms: Part VIIIScreening of some marine flora of Western coast of India. Indian J. Exp.Biol. 35, 638-643.
- Visco AC, Pesando D and Baby C (1987) Antibacterial and antifungal properties of some marine diatoms in culture. Bot. Mar. 30, 41-45.
- Young-Won Chin, Marcy J, Balunas, Hee Byung Chai and Douglas Kinghorn A (2006) Drug discovery from natural sources. The AAPS J. 8: (2), 28- 39.
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