Total views : 1517

In-vitro Callus Induction and Shoot Regeneration in Ipomoea obscura (l.): Potent Indian Medicinal Plant

Affiliations

  • Department of Botany, RTM, Nagpur University, Nagpur 440033, India
  • SFS Centre for Biotechnology, St. Francis De Sales College, Seminary Hills, Nagpur 06, India

Abstract


Callus of the leaves, node and bud of Ipomoea obscura (L) was initiated on MS basal media supplemented with various combinations of auxins 2,4- dichlorophenoxyacetic acid (2,4-D) and naphthalene acetic acid with cytokines kinetin and benzyl amino-purin (BAP). Callus initiation was observed in all media but with varied mass. Highest percentage of callus response was obtained in combination of 2,4-D (0.8 mg/L) with kinetin (0.8mg/L); NAA (0.2mg/L) with BAP(0.2 mg/L); NAA (0.8 mg/L) with kinetin (0.8 mg/L) for explants leaves, node and bud, respectively. The callus was white in 2,4-D- and green in NAA- supplemented MS media. An efficient micropropogation protocol was developed for medicinal plant Ipomoea obscura (L.) by in-vitro culture of nodal part of mature plant. The MS media supplemented with 0.8 mg/L NAA with 0.8 mg/L kinetin induced three shoots per node in an average and was best for axillary bud proliferation.

Keywords

Ipomoea Obscura, Tissue Culture, Medicinal Plant

Full Text:

 |  (PDF views: 423)

References


  • Atal CK and Kapur BM (1982 a) Cultivation and Utilization of Aromatic plants. Regional research Laboratory CSIR, Jammu Tawi.
  • Atal CK and Kapur BM (1982 b) Cultivation and Utilization of Medicinal plants. Regional research Laboratory CSIR, Jammu Tawi.
  • Bitton NL, Addison B (1913) An Illustrated flora of the Northern United state and Canada, Vol 3, 2nd Ed. , Pover Publications Inc., pp: 43-45.
  • Christopher B and Judith DZ (1997) American Horticultural Society A-Z Encyclopedia of Garden Plants DK publishing, pp: 555-556.
  • Chritophe W, Pharm D, Humana press (2002) Ethnopharmacology of medicinal plants: Asia and the Pactic, 1st Vol, 69, pp: 91.
  • Datta PC (1993) Biotechnology and Tissue culture of some medicinal plants. InGovil JN, SInghVK, Hashmi S (eds) Glimpses in plant research, Medicinal plant, New Vistas of research Part II Vol XI: 337-342.
  • Eckart E (2008) Solanaceae and Convolvulaceae – secondary metabolite, vol-1st, pp 637.
  • Feyissa T, Welander M and Nagesh L (2005) Micropropagation of Hagenia abyssinica : A multipurpose tree. Plant Cell Tissue Organ Cult. 80, 119- 127.
  • Hamilton AC (2004) Medicinal Plant, Conservation and livelihood. Biodiversity. 3(8), 1477-1517. 10. Laksmi B and Vimala V (2000) Nutritive value of dehydrated green leafy vegetables. J. food Sic. Tech., 37-465-474.
  • Pramod T, Tilok SR and Suman K (2007) Micropropogation of coptis teeta wall-Threatened medicinal plant of Arunachal Pradesh, India. Indian J. Biotechnol. 6, 280-282.
  • Rajeshwari and Kailash paliwal (2008) callus induction and plant regeneration of red sanders (pterocapus santhalinus L.) form cotyledonary node. Indian J. Biotechnol. 7, 541-546.
  • Shahina A (1994) Handbook of Arabian medicinal plants, CRC publication, pp: 90.
  • Singh MP and Himadra P (2005) Medicinal Herbs with their formulations.2nd Vol, pp.503.
  • Verdcourt B (1963) Flora of tropical east Africa (East African Herbarium) 1st Vol, pp.90.

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.