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In-vitro Callus Induction and Shoot Regeneration in Ipomoea obscura (l.): Potent Indian Medicinal Plant
Callus of the leaves, node and bud of Ipomoea obscura (L) was initiated on MS basal media supplemented with various combinations of auxins 2,4- dichlorophenoxyacetic acid (2,4-D) and naphthalene acetic acid with cytokines kinetin and benzyl amino-purin (BAP). Callus initiation was observed in all media but with varied mass. Highest percentage of callus response was obtained in combination of 2,4-D (0.8 mg/L) with kinetin (0.8mg/L); NAA (0.2mg/L) with BAP(0.2 mg/L); NAA (0.8 mg/L) with kinetin (0.8 mg/L) for explants leaves, node and bud, respectively. The callus was white in 2,4-D- and green in NAA- supplemented MS media. An efficient micropropogation protocol was developed for medicinal plant Ipomoea obscura (L.) by in-vitro culture of nodal part of mature plant. The MS media supplemented with 0.8 mg/L NAA with 0.8 mg/L kinetin induced three shoots per node in an average and was best for axillary bud proliferation.
Ipomoea Obscura, Tissue Culture, Medicinal Plant
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