Total views : 1287
In vitro Propagation and Cell Suspension Culture of Callistemon citrinus L.
The bottle brush tree, Callistemon citrinus is a shrub that is nearing extinction. An attempt to revive this plant and propagate it in vitro has been performed for large scale production. Calli cultured on MS medium with different combinations of cytokinins and auxins viz. 1.0 mg/l Kinetin along with 1.0 mg/l IAA; 1.5 mg/l Kinetin along with 1.0 mg/l IAA; 1.0 mg/l Kinetin along with 1.0 mg/l IBA, and 2.0 mg/l Kinetin along with 1.5 mg/l IBA. All combinations were very effective in inducing callus. 0.5 mg/l kinetin along with 0.5 mg/l IAA was very effective for shoot regeneration and shoot elongation. The developed shoots when transferred to MS medium, supplemented with 1.5mg/l IBA, shows root development within a week. Axillary bud proliferation is also observed in the explants inoculated in MS medium supplemented with combinations of kinetin and IAA.
Callistemon citrinus, Indole Acetic Acid (IAA), Indole Butyric Acid ((IBA), Benzyl Amino Purine(BAP)
- Brophy JJ, Goldsack RJ, Forster PI, Craven LA and Lepschi BJ (1998) The leaf essential oils of the Australian members of the genus Callistemon (Myrtaceae). J. Essen. Oil Res. 10(6), 595- 606.
- Chistokhodova N, Nguyen C, Calvino T, Kachirskia I and Cunningham G (2002) Antithrombin activity of medicinal plants from central Florida. J. Ethnopharma. 81(2), 277-280.
- Dutta BK, Karmakar S, Naglot A, Aich JC and Begam M (2007) Anticandidial activity of some essential oils of a mega biodiversity hotspot in India. Mycoses. 50 (2), 121-124.
- Mahmoud II, Moharram FA, Marzouk MS, Linscheid MW and Salch MI (2002) Polyphenolic constituents of Callistemon lanceolatus leaves. Pharmazie. 57(7), 494-496.
- Lin Che, Pei FU, Wu Lin-sen and Shen Pei-fu (2005) Micropropagation of Callistemon viminalis. J. Fuji Fore. Sci. Technol. 32(1), 52-54.
- Sharma RK, Kotoky R and Bhattacharya PR (2006) Volatile oil from the leaves of Callistemon lanceolatus D.C. grown in northeastern India. Flav. Frag. J. 21(2), 239-240.
- Stanaland BF, Gennaro RN, Sweeney MJ and White RS (1986) Isolation and characterization of cross-reactive allergenic components in Callistemon citrinis and Melaleuca quinquenervia pollen by trans-blot enzymelinked crossed immunoelectrophoresis. Arch Allergy Appl. Immunol. 80(3), 278-284.
- Sudhakar M, Rao CHV, Rao AL and Raju DB (2004) Antinociceptive and anti-inflammatory effects of the standardized oil of Indian Callistemon lanceolatus leaves in experimental animals. Acta Pharmaceutica. Turcica 46(2), 131-139.
- Vogler B, Klaiber I, Roos G, Walter CU, Hiller W, Sandor P and Kraus W (1998) Combination of LC-MS and C-NMR as a tool for the structure determination of natural products. J. Natural Products. 61, 175–178.
- Cheng hou Wu, Feng Yi-min, Ye Zhen-hua, Zhu Chun and Long Li-ping (2007) Callus induction and plant regeneration in Callistemon rigidus (J). Nonwood Forest Res. 27(6),
- Ying Wang (2008) Needs for new plant-derived pharmaceuticals in the post-genome era: an industrial view in drug research and development. Phytochem. Rev. 7, 395–406.
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 3.0 License.