Total views : 411
Investigations on the Kinetics of Acid Catalyzed Hemicellulose Hydrolysis Derived from Waste Rice Straw for the Production of Bio-Ethanol
Objectives: To understand the monophasic kinetic behavior of hemicellulose hydrolysis and determination of thermodynamic parameters involved in dilute acid pretreatment by tartaric acid. Methods/Statistical Analysis: Biomass samples from thatched roofs were collected and analyzed for cellulose, hemicellulose and lignin through Neutral Detergent Fibre (NDF), Acid Detergent Fibre (ADF) and Acid Detergent Lignin (ADL) methods. The ground biomass was hydrolysed with tartaric acid, saccharified applying cellulase, xylanase at different time/agitation speed for obtaining reducing sugar and finally fermented for ethanol yield. The kinetics of hemicellulose hydrolysis was critically studied in the present work. Findings: The maximum yield of xylose and reducing sugars after hydrolysis and saccharification at optimum conditions were 134 mg/g and 494.8 mg/g using tartaric acid as hydrolyzing media. Pichia stipitis was very effective for conversion of pentoses and hexoses during fermentation. The maximum ethanol yield of 25.1% using tartaric acid was obtained after fermentation. Overall, the present works depicts the introduction of a new hydrolytic agent, tartaric acid and a comprehensive view of the complete bio processing of waste rice straw to bioethanol. The amount of ethanol yield was from 17% to 25.1%. It was noticed that the ethanol content was directly proportional with the increase in age of the samples. An increasing trend of ethanol with time was visible until 9th hour. High ethanol yield was obtained in this study since Pichia stipitisstrain could utilize both hexose sugars and pentose sugars together. Applications/Improvements: Thatched rice straw roof can be better utilized for ethanol making with natural degradation of lignin due to aging instead of increasing environment pollution using significantly less synthetic enzymes.
Bioethanol, Hydrolysis, Kinetics, Thermodynamics, Waste Rice Straw
- Demirbas A. Biomass resource facilities and biomass conversion processing for fuels and chemicals. Energy Conversion and Management. 2001; 42(11):1357-78. Crossref
- Stocker M. Biofuels and biomass‐to‐liquid fuels in the biorefinery: Catalytic conversion of lignocellulosic biomass using porous materials. Angewandte Chemie International Edition. 2008; 47(48):9200-11. Crossref
- Patel AK, Singhania RR, Pandey A. Biofuels from biomass.Novel Combustion Concepts for Sustainable Energy Development: Springer; 2014. p. 25-44.
- Valentine J, Clifton‐Brown J, Hastings A, Robson P, Allison G, Smith P. Food vs. fuel: The use of land for lignocellulosic ‘next generation’ energy crops that minimize competition with primary food production. GCB Bioenergy. 2012; 4(1):1-19. Crossref
- Zamora R, Sanchez CJ. Production of an acid extract of rice straw. Acta Cientifica Venezolana. 1994; 46(2):135-9.
- Garrote G, Domí H, Parajo JC. Autohydrolysis of corncob: Study of non-isothermal operation for xylooligosaccharide production. Journal of Food Engineering. 2002; 52(3):211-8.Crossref
- Saha BC. Hemicellulose bioconversion. Journal of Industrial Microbiology and Biotechnology. 2003; 30(5):279-91.Crossref
- Rafiqul I, Sakinah AM. Processes for the production of xylitol: A review. Food Reviews International. 2013; 29(2):12756. Crossref
- Zhang M, Eddy C, Deanda K, Finkelstein M, PicataggioS. Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis. Science. 1995; 267(5195):240-3. Crossref
- Ho NW, Chen Z, Brainard AP. Genetically engineered Saccharomycesyeast capable of effective cofermentation of glucose and xylose. Applied and Environmental Microbiology.1998; 64(5):1852-9.
- Ingram L, Gomez P, Lai X, Moniruzzaman M, Wood B, Yomano L, et al. Metabolic engineering of bacteria for ethanol production. Biotechnology and Bioengineering. 1998; 58(2‐3):204-14. Crossref
- Horitsu H, Yahashi Y, Takamizawa K, Kawai K, Suzuki T, Watanabe N. Production of xylitol from D‐xylose by candida tropicalis: Optimization of production rate. Biotechnology and Bioengineering. 1992; 40(9):1085-91. Crossref
- Lee Y, Iyer P, Torget R. Recent progress in bioconversion of lignocellulosics. Recent Progress in Bioconversion of Lignocellulosics.1999.
- Jacobsen SE, Wyman CE, editors. Cellulose and hemicellulose hydrolysis models for application to current and novel pretreatment processes. 21st Symposium on Biotechnology for Fuels and Chemicals; 2000.
- Wyman CE, Dale BE, Elander RT, Holtzapple M, Ladisch MR, Lee Y. Coordinated development of leading biomass pretreatment technologies. Bioresource Technology. 2005; 96(18):1959-66. Crossref
- Saeman JF. Kinetics of wood saccharification-hydrolysis of cellulose and decomposition of sugars in dilute acid at high temperature. Industrial & Engineering Chemistry. 1945; 37(1):43-52. Crossref
- Elander R, Hsu T. Processing and economic impacts of biomass delignification for ethanol production. Applied Biochemistry and Biotechnology. 1995; 51(1):463-78. Crossref
- Pessoa Jr A, Mancilha I, Sato S. Acid hydrolysis of hemicellulose from sugarcane bagasse. Brazilian Journal of Chemical Engineering. 1997; 14(3). Crossref
- Dumitriu S. Polysaccharides: Structural diversity and functional versatility. CRC Press; 2004. Crossref
- Goering HK, Van Soest PJ. Forage fiber analyses (apparatus, reagents, prcedures, and some applications). USDA Agr Handb. 1970.
- Updegraff DM. Semimicro determination of cellulose in biological materials. Analytical Biochemistry. 1969; 32(3):420-4. Crossref
- Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 1959; 31(3):426-8. Crossref
- Trinder P. Micro-determination of xylose in plasma. Analyst.1975; 100(1186):12-5. Crossref
- Aguilar R, Ramırez J, Garrote G, Vazquez M. Kinetic study of the acid hydrolysis of sugar cane bagasse. Journal of Food Engineering. 2002; 55(4):309-18. Crossref
- Li X, Converse AO, Wyman CE. Characterization of molecular weight distribution of oligomers from autocatalyzed batch hydrolysis of xylan. Applied Biochemistry and Biotechnology. 2003; 107(1-3):515-22. Crossref
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 3.0 License.