Indian Journal of Science and Technology
Year: 2015, Volume: 8, Issue: 27, Pages: 1-10
Natalia Andreevna Krivova1*, Olga Borisovna Zaeva1 , Irina Vladimirovna Sukhodolo2 , Ivan Vasilevich Milto2 and Valeria Aleksandrovna Grigorieva1
1 Laboratory of Experimental Physiology, Institute of Biology and Biophysics, Tomsk State University, 36 Lenina Ave, Tomsk, Russian Federation; [email protected]
2 Department of Morphology and General Pathology, Siberian State Medical University, Moskovsky Trakt 2, Tomsk, Russian Federation
Aging of the body is accompanied by a gradual development of the oxidative stress. However, this process has different rates in various systems of the body; so, it seems interesting to compare the levels of oxidative stress in different systems during aging. The luminol-dependent chemiluminescence method with the Fenton reaction was used to investigate the antioxidant capacity. The light sums of chemiluminescence for antioxidant capacity and free radicals of the oxidized molecules were determined to find the ratio of these sums (redox ratio) in the indicated tissues. Changes in the light sums of chemiluminescence for antioxidant capacity and free radicals in the internal organs generally agree with the commonly accepted ideas on the role of oxidative stress in aging. All the studied parts of the brain showed a substantial increase in the light sums of chemiluminescence for antioxidant capacity and free radicals. A comparison of the level of free radicals and the antioxidant capacity has revealed differences in their ratio in the internal organs, blood plasma and brain parts during aging and different levels of oxidative stress. Results of the comparative study gave grounds to suggest that the brain has some other components with antioxidant capacity, which are absent in the heart, lungs, liver and skeletal muscles. Such components in the brain may be glycan, which possess the antioxidant capacity and are widely represented in the brain parts.
Keywords: Aging, Antioxidant Capacity, Brain, Internal Organs
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