Indian Journal of Science and Technology
Year: 2009, Volume: 2, Issue: 5, Pages: 55-57
S. Karthikeyan, R. Prasad, T.S. Mahendran, K. Rajagopal and V. Ravendran1
Department of Biotechnology, Vel’ s University, Pallavaram, Chennai 600 117, India.
1Department of Botany, Madras Christian College, Chennai 600 059, India.
*Author for the correspondence:
Department of Biotechnology,
Vel’ s University, Pallavaram, Chennai 600 117, India.
E-mail: [email protected]
This study reports a simple micropropagation protocol and thereby rapid multiplication of the useful medicinal plant- Scoparia dulcis L. Single node explants were inoculated on basal MS medium containing 3% (w/v) sucrose, supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), kinetin (KN), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and Naphthalene acetic acid (NAA) for direct plant regeneration. Maximum numbers of shoot (~22) were observed on the medium containing 0.5 mg/l BAP and 0.25 mg/l IAA after four weeks of culture. Regenerated shoots were separated and rooted on half strength MS medium supplemented with 0.5 mg/l of IBA alone for three weeks. Simultaneous regeneration of shoots and roots and in vitro flowering were achieved from the nodal explants on MS medium supplemented with 0.5 mg/l KN and 2.0 mg/l IAA. Well-developed complete plantlets were transferred on to specially made plastic cup containing soil rite. Acclimatized plantlets were successfully grown in garden soil.
Keywords: Scoparia dulcis, nodal explants, micropropagation, plant tissue culture.
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