Fermented Neem (Azadirachta indica) leaves-metal nanoparticles and their insecticidal properties against Bactrocera dorsalis (Hendel)

Danila S Paragas; Kathlia D C Cruz; Elaida R Fiegalan

doi:10.17485/IJST/v14i17.631

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Indian Journal of Science and Technology

DOI: 10.17485/IJST/v14i17.631

Year: 2021, Volume: 14, Issue: 17, Pages: 1338-1346

Original Article

Fermented Neem (Azadirachta indica) leaves-metal nanoparticles and their insecticidal properties against Bactrocera dorsalis (Hendel)

Danila S Paragas^1,^2,*, Kathlia D C Cruz¹, Elaida R Fiegalan³

¹School of Chemical, Biological and Materials Engineering and Sciences, Mapua University,
Intramuros, Manila, Philippines
²Department of Chemistry, College of Science, Central Luzon State University, Science City of
Muñoz, 3120, Nueva Ecija, Philippines
³Department of Crop Protection, College of Agriculture, Central Luzon State University,
Science City of Muñoz, 3120, Nueva Ecija, Philippines

*Corresponding Author
Email: [email protected]

Received Date:14 April 2021, Accepted Date:29 April 2021, Published Date:12 May 2021

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: To investigate the potential of nanoparticles synthesized from neem leaves in pest management for Bactrocera dorsalis. Methods: This study involves extracting the insecticidal properties of neem (Azadirachta indica) by fermenting air-dried leaves with rice wash. The crude fermented neem extract (FNE) was applied as a biopesticide against male B. dorsalis in an improvised olfactometer. The remaining crude extract was utilized in the green synthesis of silver nanoparticles (AgNP) and copper nanoparticles (CuNP). The insecticidal activities of FNE and the extracts with AgNP and CuNP were tested against B. dorsalis. Findings: The experimental treatments 100% FNE and 20% FNECuNP have the same effect as the positive control, causing the death of adult male fruit fly of 83.33% mortality rate after 24 h. The treatment 20% FNE-AgNP showed a higher mortality rate, 100%, after 24 h. Likewise, the results of the larvicidal activities infer that the most effective treatment with a mortality rate of 100% after 24-h exposure is the extract with silver nanoparticles. Moreover, the neem extract, FNE-AgNP, and FNE-CuNP exhibited ovicidal properties, suppressing the development of eggs into third instar larvae. Fermented neem extract can be used to formulate biopesticide enhanced with nanoparticles for controlling B. dorsalis. Novelty: This study showed that the fermented neem leaves extract using rice wash is effective in the green synthesis of silver and copper nanoparticles. The prepared biopesticide metal nanoparticles can be used in the management of B. dorsalis.

Keywords: Silver nanoparticles; Copper nanoparticles; Insecticidal; Bactrocera dorsalis; Azadirachta indica

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© 2021 Paragas et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)