Indian Journal of Science and Technology
Year: 2010, Volume: 3, Issue: 1, Pages: 67-69
R.S. David Paul Raj1 , Sheena Michael Morais2 and K. Gopalakrishnan1*
1 Center for Research and Development, PRIST University, Thanjavur, Tamilnadu, India.
2 Dept. of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, India.
The bottle brush tree, Callistemon citrinus is a shrub that is nearing extinction. An attempt to revive this plant and propagate it in vitro has been performed for large scale production. Calli cultured on MS medium with different combinations of cytokinins and auxins viz. 1.0 mg/l Kinetin along with 1.0 mg/l IAA; 1.5 mg/l Kinetin along with 1.0 mg/l IAA; 1.0 mg/l Kinetin along with 1.0 mg/l IBA, and 2.0 mg/l Kinetin along with 1.5 mg/l IBA. All combinations were very effective in inducing callus. 0.5 mg/l kinetin along with 0.5 mg/l IAA was very effective for shoot regeneration and shoot elongation. The developed shoots when transferred to MS medium, supplemented with 1.5mg/l IBA, shows root development within a week. Axillary bud proliferation is also observed in the explants inoculated in MS medium supplemented with combinations of kinetin and IAA.
Keywords: Callistemon citrinus, Indole Acetic Acid (IAA), Indole Butyric Acid ((IBA), Benzyl amino purine(BAP).
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