• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2015, Volume: 8, Issue: Supplementary 1, Pages: 1-5

Original Article

Modulation of Oral Epithelial Cell Properties by Insulin


The cell culture environment regulates epithelial cell properties by changing a number of signaling pathways and ­intercellular interactions among different cell types. Culture medium contains many components that stimulate cell phenotypic changes. Many studies have reported modulating cells by altering the culture environment. Changes in culture conditions can induce the epithelial to mesenchymal transition and the mesenchymal to epithelial transition. Therefore, microenvironmental interactions between the culture medium and cells entail cytoskeletal remodeling. We studied the characteristics of oral epithelial cells according to insulin concentration, which is used as a cell culture supplement because it delays apoptosis and promotes proliferation. A high insulin concentration turned fibroblastic epithelial cells into a cobblestone-like morphology, shortened cell-to-cell distance, and contributed to increase cell growth. These results ­provide data on the effect of insulin in oral epithelial cells under in vitro culture conditions.
Keywords: EMT, Environment, Insulin, MET, Morphology


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