Indian Journal of Science and Technology
Year: 2017, Volume: 10, Issue: 34, Pages: 1-17
Divya Vishambhar Kumbhakar1 , Animesh Kumar Datta1*, Debadrito Das1 , Venu Prabha Sahu2 , Sukhdeo B. Barbuddhe2 , Suraj Sharma2 , Bapi Ghosh1 and Ankita Pramanik1
1Department of Botany, Cytogenetics, Genetics and Plant breeding Section, Kalyani University, Kalyani, Nadia - 741235, West Bengal, India; [email protected], [email protected], [email protected], [email protected], [email protected] 2Indian Council of Agricultural Research-National Institute of Biotic Stress Management, Baronda, Raipur - 493225, Chattisgarh, India; [email protected], [email protected], [email protected]
*Author for the correspondence:
Animesh Kumar Datta
Department of Botany, Cytogenetics, Genetics and Plant breeding Section, Kalyani University, Kalyani, Nadia - 741235, West Bengal, India; [email protected]
Objectives: Use of nanoparticles (Cu- and CdS-NPs) in mineralization of azo dyes (methyl red and malachite green) for waste water management and effectivity against serotypes of Listeria monocytogenes and Salmonella typhimurium. Methods: To study azo-dyes mineralization potentiality, dye-NPs reaction mixtures are analyzed using UV-vis near infra-red spectroscopy, HPLC and UPLC-ESI-QTOF-MS. Antimicrobial effectivity of NPs is studied following quantification of inhibition zone from disc diffusion assay. NPs mediated bacterial cell cycle inhibition is analyzed following flow cytometry. Findings: The proposed MG degradation pathways using Cu- and CdS- NPs are pioneering reports. Dye mineralization efficiency of Cu-NPs is found to be higher than CdS-NPs. On the basis of analysis of reaction intermediates (using HPLC, ESI-QTOF-MS), separate dye degradation pathways are proposed. Disc diffusion assay reveals antimicrobial effectivity of NPs for controlling human pathogenic bacteria. Flow cytometry can be used as efficient tool for ascertaining NPs mediated microbial growth inhibition. Application: Photocatalytic potentiality of the prepared NPs can be effectively used for removal of azo-dyes contaminants from waste water. NPs can also be effectively used against studied human pathogenic bacteria for drug designing.
Keywords: Azo-dyes; Antimicrobial Potentiality, Cu- and CdS-NPs, FACs, Photocatalysis
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