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Genetic Diversity and Relationships among Sugarcane (Saccharum sp.) from Thailand Revealed by RAPD and AFLP Markers
Objective: The objective of the present research was to evaluate the genetic diversity and relationships of sugarcane (Saccharum sp.) that collected from the sugarcane germplasm collection, Thailand. Methods: Genetic diversity of sugarcane was detected by DNA sequencing, Random Amplified Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) markers. Findings: The trnL- trnF region of the chloroplast genome was amplified for 26 cultivars with C and F primers that the fragment was approximately 950 base pairs (bp). The taxonomic status and relationships of these sequences are not resolved. RAPD marker was used to study genetic variation and relationships among 56 sugarcane varieties and their hybrids. Ninety-six RAPD primers were screened and nine primers of them produced reproducible and recordable bands were used in analysis. The eighty-eight polymorphic bands (96.70%) were detected and genetic similarity was 0.657. The best five AFLP primer combinations were selected and generated 187 polymorphic bands (80.26%). Comparing the results from the RAPD and AFLP markers in 19 sugarcane cultivars, the genetic similarity ranging from 0.582 to 0.927, with the average of 0.756 for AFLP and ranging from 0.449 to 0.865, with the average of 0.657 for RAPD that demonstrating a low level of genetic diversity. For dendrogram analysis, AFLP marker showed itself to be more efficient at discriminating sugarcane cultivars. However, the genetic relationship of the samples slightly correlated with sucrose content. Application: RAPD and AFLP markers can be used to help plant breeder to select cultivars for future sugarcane improvement programs.
AFLP, Genetic Diversity, RAPD, Saccharum sp., Sugarcane
- Nawaz S, Khan FA, Tabasum S, Zakria M, Saeed A, Iqbal MZ. Phylogenetic relationship among Saccharum clones in Pakistan revealed by RAPD markers. Genetics and Molecular Research. 2010; 9(3):1673-82. Crossref. PMid:20799164
- Daniels J, Roach BT. Taxonomy and evolution. Amsterdam: Elsevier Science: Heinz DJ, editors. Sugarcane improvement through breeding. 1987. Crossref.
- Tew TL. New York: Elsevier: World sugarcane variety censusYear 2000. Improvement through breeding. 2003.
- Roach BT. Origin and improvement of the genetic base of sugarcane. Proceedings of the Australian Society of Sugar Cane Technologists, 1989; 11:34-47.
- Tabasum S, Khan FA, Nawaz S, Iqbal MZ, Saeed A. DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA. Genetics and Molecular Research. 2010; 9(1):471-83. Crossref. PMid:20391332
- Rodríguez M, Hernández I, Coto O, Canales E, Cornide MT. RFLP and AFLP polymorphism analysis in sugarcane varieties. Revista CENIC Ciencias Biologicas. 2003; 34(3):121-8.
- Selvi A, Nair NV, Singh NK. AFLP analysis of the phonetic organization and genetic diversity in the sugarcane complex, Saccharum and Erianthus. Genetic Resource Crop Evolution. 2006; 53:831-42. Crossref.
- Khaled KAM, El-Demardash IS, Amer EAM. Genetic polymorphism among some sugarcane germplasm collections as revealed by RAPD and ISSR analyses. Life Sci J. 2015; 12(3):159-67.
- Singh RK, Singh RB, Singh SP, Sharma ML. Identification of sugarcane microsatellites associated to sugar content in sugarcane and transferability to other cereal genomes. Euphytica. 2011; 182:335-54. Crossref.
- Chaiyabut A, Poeaim S, Poeaim A, Distabanjong K. Genetic diversity analysis of sugarcane (Saccharum sp.) in Thailand using RAPD technique. International Journal of Agricultural Technology. 2014; 10(1):159-65.
- Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 1990; 19(1):11-5.
- Taberlet P, Gielly L, Pautou G, Bouvet J. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology. 1991; 17:1105-9. Crossref. PMid:1932684
- William JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SU. DNA polymorphism amplified by arbitrary primer are useful as genetic markers. Nucleic Acids Research. 1990; 8:6531-5. Crossref.
- Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zebeau M. AFLP: A new technique for DNA finger printings. Nucleic Acids Research. 1995; 23(21):4407-14. Crossref. PMid:7501463 PMCid:PMC307397
- Besse P, Taylor G, Carroll B, Berding N, Burner N, Burner D, McIntyre CL. Assessing genetic diversity in sugarcane germplasm collection using an automated AFLP analysis. Genetica. 1998; 104(2):143-53. Crossref. PMid:16220373
- Zhu JR, Zhou H, Pan YB, Lu X. Genetic variability among the chloroplast genomes of sugarcane (Saccharum spp) and its wild progenitor species Saccharum spontaneum L. Genetics and Molecular Research. 2014; 13(2):3037-47. Crossref. PMid:24615073
- Welker CAD, Souza-Chies TT, Longhi-Wagner HM, Peichoto MC, McKain MR, Kellogg EA. Phylogenetic analysis of Saccharum s.l. (Poaceae; Andropogoneae), with emphasis on the circumscription of the South American species. American Journal of Botony. 2015; 102(2):248-63. Crossref. PMid:25667078
- Hapsoro D, Warganegara HA, Utomo SD, Sriyani N, Yusnita Y. Genetic diversity among sugarcane (Saccharum officinarum L.) genotypes as shown by randomly amplified polymorphic DNA (RAPD). Agrivita. 2015; 37(3):247-57. Crossref.
- Pan YB, Burner DM, Legendre BL, Grisham MP, White WH. An assessment of the genetic diversity within a collection of Saccharum spontaneum L. with RAPD-PCR. Genetic Resource Crop Evolution. 2004; 51(8):895-903. Crossref.
- Lima ML, Garcia AA, Oliveira KM, Matsuoka S, Arizono H, De Souza CL, De Souza AP. Analysis of genetic similarity detected by AFLP and coefficient of parentage among genotypes of sugarcane (Saccharum spp.). Theoretical and Applied Genetics. 2002; 104(1): 30-38. Crossref. PMid:12579425
- Rodriguez HAM, Castillo CMA, Flores BEP. Genetic diversity of the most important sugarcane cultivars in Mexico. E-Gnosis. 2005; 3(1):1-10.
- Selvi A, Nair NV, Noyer JL, Singh NK, Balasundaram N, Bansal KC, Koundal KR, Mohapatra T. Genomic constitution and genetic relationship among the tropical and subtropical Indian sugarcane cultivars revealed by AFLP. Crop Science. 2005; 45(5):1750-7. Crossref.
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