Total views : 1158
A Comparative Study of Pre and Post Vaccinated Sera Samples of Bovine Using FTIR Spectral Analysis
This study provides scope for spectroscopic method that can be used as in-vitro test to assess the potent of vaccination instead of conventional in-vivo test. India tops in cattle wealth as well as in milk production in the world. Livestock are to be regularly vaccinated to protect them from many infectious and contagious diseases. We investigated the changes in protein and lipids levels in the pre and post vaccinated sera samples of bovine using FTIR spectral analysis. Two groups of seven animals were vaccinated with Haemorrhagic Septicaemia Vaccine (HSV) and Black Quarter Vaccine (BQV). Pre and post vaccinated sera samples of cattle were collected and tested using Fourier Transform Infra Red (FTIR) spectrometer. Spectroscopic method of blood analysis is an alternate technique to the clinical method since they require fewer samples and provide more information. The variation observed in peaks indicating the change in protein and lipids levels in the animals was due to introduce of antigens. Compared to ELISA, spectral analysis is cost effective test besides it requires small amount of sample for analysis and conforms to bioethics. In vivo challenge test can be replaced once this procedure is standardized which can satisfy the CPCSEA-"Committee for the Purpose of Control and Supervision on Experiments on Animal"- which imposes stringent regulations to use animal for experiments.
Cattle, Livestock, Vaccination, Spectroscopy, FTIR Spectrometer
- Christopher Hodgson J, Anna Finucane, Mark P. Dagleish, Saeed Ataei, Roger Parton and John G. Coote (2004) Efficacy of Vaccination of Calves against Hemorrhagic Septicemia with a Live aroA Derivative of Pasteurella multocida B:2 by two different routes of administration. Infection & Immunity. 73 (3), 1475-1481.
- Gunasekaran .S and T.S.Renuga Devi (2008) Analysis of Hyperlipidemic Patients Blood Samples – Spectral and Statistical Implications. Asian J. Clinical Cardiology, 10 (10),14-19.
- Gunasekaran S and Sankari G (2004) FTIR and UV-Visible spectral study on normal and diseased blood samples. Asian J. Chem. 16, 1779-1786.
- Heise HM (2008) Biomedical vibrational spectroscopy- Technical advances. In: Biomedical Vibrational Spectroscopy. Lasch P & Kneipp J (Eds.), Wiley. pp: 9-37.
- Lingathurai S, Vellathurai P, Ezil Vendan S and Alwin Prem Anand A (2009) A comparative study on the microbiological and chemical composition of cow milk from different locations in Madurai, Tamil Nadu. Indian J.Sci.Technol. 2 (2), 51-54. Domain site: http://www.indjst.org.
- Renuga Devi TS, Gunasekaran S, Wesley Hudson J and Sarone Angelah Joybell I (2009) Analysis on renal failure patients blood samples: characterization and efficacy study. Indian J.Sci.Technol. 2 (2), 46-50. Domain site: http://www.indjst.org.
- Shaw RA, Kotowich S, Leroux M and Mantsch HH (1998) Multianalyte serum analysis using mid-infrared spectroscopy. Ann. Clin. Biochem. 35, 624-632.
- Srinivasan VA, Reddy GS, Rao KA and Ueli Kihm (2001) Serological response of bovines to combined vaccine containing foot and mouth disease virus, rabies virus, Pasteurella multocida and Clostridium chauvoei antigens. Veterinarski Arhiv. 71 (1), 37-45.
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 3.0 License.