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In vitro Propagation of Endangered Medicinal Plant-Commiphora wightii

Affiliations

  • Boston College for Professional Studies, Sirol Road, Gwalior 474006, India
  • School of Studies in Botany, Jiwaji University, Gwalior-474011, India

Abstract


Present study reports in vitro multiplication and conservation of Commiphora wightii and the influence of growth regulators, location of the explant on mother plant, influence of apical dominance and the size of explant on regeneration ability. Out of the 40, single as well as combinations of BAP, Kn, NAA, IBA and GA3 used, only three combination treatments of BAP (3mg/l) with IBA (0.1-0.3mg/l) induced shoot development in node and shoot tip cultures. Further, among the three nodes, 1st node (shoot tip) performed better on BAP, IBA and GA3 supplemented medium. Commiphora wightii shows strong apical dominance, the influence of dominance was tested with four explants- shoot tip, simple node, axillary bud and node -AD. Shoot tip, simple node, and axillary bud result no shoot bud development on any concentration or combinations of BAP and/or GA3tested. Whereas, the node -ADs alone showed shoots development. Best percentage response of node-AD was observed on 2mg/l BAP+0.5mg/l GA3 (27%) followed by 1mg/l BAP+1.5mg/l GA3 (25%) medium. Further, node-AD with two nodes and two internodes performed better and survived for more than 6 weeks on the same medium without subculture as compared to node-AD of 0.5-0.8 cm in size with single node. Regenerated shoots transferred to MS + activated charcoal medium after dipping in NAA, IAA or IBA (200ppm) solutions , showed root development within 20 days of culture with 24 - 26% response. All the regenerated plants were successfully transferred to field conditions. Thus, the paper reports the successful in vitro propagation of Commiphora wightii.

Keywords

Commiphora wightii, Endangered, Medicinal Plant, in vitro Propagation, Tissue Culture, India

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References


  • Barve DM and Mehta AR (1993) Clonal propagation of mature elite tree of Commiphora wightii. Plant Cell Tiss. Org. Cult. 35, 237-244.
  • Christopher T and Rajam MV (1996) Effect of genotype, explant and medium on in vitro regeneration of red pepper. Plant Cell Tiss. Org. Cult. 46, 245-250.
  • FRLHT (2006) A report on threat assessment for prioritized medicinal plant species of Madhya Pradesh, Coordinate by Foundation of Revitalization of Local Health Tradition and Madhya Pradesh State Biodiversity Board.
  • Fari M and Czako M (1981) Relationship between position and morphogenetic response of pepper hypocotyls explants cultured in vitro. Scientia Horticulturae. 1, 207-213.
  • Gujral ML, Sareen K, Tangari KK, Amma MK and Roy AK (1960) Anti-arthritic and anti-inflammatory activity of gum guggul (Balsamodendron mukul Hook). Indian J. Physiol. Pharmacol. 267-273.
  • IUCN (2010) IUCN Red list of threatened species. version 2010.2
  • Kant T, Tomar UK, Prajapati S and Parmar AK (2010) In vitro propagation as a viable conservation strategy for Commiphora wightii, an endangered medicinally important desert tree, India. Conservation Evidence. 94-99.
  • Kasera PK and Mohammed S (2007) Critically threatened plants of the Thar desert, India – Their utilization and conservation strategies. J. Econ.Taxon. Bot. 31(3), 579-583.
  • Kirtikar KR, Basu BD (1987) Indian Medicinal Plants. Vol-III, International Book Distributors & Publ., Dehradun, India. pp:1815-1817.
  • Kumar S, Suri SS, Sonie KC and Ramawat KG (2004) Development of `biotechnology for Commiphora wightii: A potent source of natural hypolipidemic and hypocholesterolemic drug. In: Plant biotechnology and molecular markers. Anamaya Publ., New Delhi, India. pp: 130-143.
  • Kumar S, Mathur M, Jain AK and Ramawat KG (2006) Somatic embryo proliferation in Commiphora wightii and evidence for guggulsterone production in culture. Indian J. Biotechnol. 5, 217-222.
  • Mathur M, Jain AK and Ramawat KG (2007) Optimization of guggulsterone production in callus cultures of Commiphora wightii (Arnott.) Bhandari. Indian J. Biotechnol. 6, 525-531.
  • Mitsukuria K, Moria G, Johkana M, Shimadaa KI, Mishibaa T, Morikawaa T and Masayuki Oda A (2009) Effect of explant position and dark treatment on bud formation in floret culture of Ponerorchis graminifolia Rchb. F.Scientific Horticulture. 121(2), 243-247.
  • Mok SH and Norzulaani K (2007) Troubleshooting for recalcitrant bud formation in Capsicum Annuum var. Kulai. Asia Pacific J. Mol. Biol. Biotechnol.15 (1), 33-38.
  • Murashige T and Skoog F (1964) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 25, 135-166.
  • Nityanand S and Kapoor NK (1971) Hypocholesterolemic effect of Commiphora mukul resin. Indian J. Exp. Biol. 9, 376-377.
  • Nohr LA, Rasmussen LB and Straand J (2009) Resin from the mukul myrrh tree, guggul, can it be used for treating hypercholesterolemia. A randomized, controlled study. Complementary Therapies in Medicine. 17, 16-22.
  • Pereira AMS, Moro JR, Cerdeira RMM and Franca SC (1995) Effect of phytoregulators and physiological characteristic of the explants on micropropagation of Maytenus ilicifolia. Plant Cell Tiss. Org. Cult. 42(3), 295-297.
  • Prajapati AK, Upadhyaya M and Mathur AK (2010) Micro-cloning and in vitro guggulsterones production studied in Commiphora wightii (cv, Marusudha), International Symposium on Current Status and Opportunities in Aromatic & Medicinal Plants. pp:117.
  • Pullaiah T (2006) Encyclopedia of world medicinal plants Vol-2. Regency Publication, New Delhi, India, 623-625.
  • Satyavati GV (1991) A promising hypolipidemic agent from gum guggul (Commiphora wightii). Econ. Med. Plant Res. 5, 47-82.
  • Sharma P and Rajam M V (1995) Genotype, explant and position effects on organogenesis and somatic embryogenesis in eggplant (Solanum melongena L.). J. Exp. Bot. 46,135-141.
  • Soni V (2010) Efficacy of in vitro tissue culture versus stem cutting for propagation of Commiphora wightii in Rajasthan, India. Conservation Evidance. 7, 91-93.
  • Tejovathi G, Vimla Y, Bhadauria R (1996) A practical manual for plant biotechnology. CBS Publ. & Distributors, Daryagang, New Delhi.
  • Tuchila C, Jianu I, Rujescu CI, Butur M, Khoie M and Negrea I (2008) Evaluation of the antimicrobial activity of some plant extracts used as food additives. J. Food, Agri. & Environ. 6(3&4), 68-70.

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