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In vitro Shoot Micro Propagation of Medicinal Applications and Ornamental Value of Cestrum nocturnum
Background/Objectives: Cestrum nocturnum L. a night Blooming jasmine belongs to Solanaceae family widely circulated all over tropical as well as subtropical areas of the World. It is mainly popular for ornamental fragrant flowering and hedge plant but also sometimes for traditional medicinal purpose. Due to strongest smelling characteristics of the plants, it is used in many industries for making Perfumes, essential Oils, Soaps, Candles, Body Oils, etc. The existence of natural plants of economic importance are threatened due to rapid urban development, including industrialization, residential development, educational, commercial etc., reduce the land for cultivation. Hence plant tissue culture protocol may be adapted for production and utilization of economically popular plants, including C. nocturnum involving limited space and short period of time. Methods: Shoot tip explants of naturally grown C. nocturnum were excised sterilized and endued on ‘Murashige and Skoog’ (MS) medium enriched changed concentration of BA, NAA, as well as GA3 singly or in combination. Excised micro shoots were examined for root development on 0.5 MS using IBA, NAA as well as IAA separately. Findings: The highest amount of multiple bud were observed in low concentration of BA (01.50 milligram × l-1), resulted no. of shoot 4.40 as well as 4.20/explant, no. of leaves 15.40 as well as 4.20/explant as well as size of different shoot 5.360 as well as 4.860 cm. The concentration of IBA and IAA were found to be best for root formation in micro shoots (13.20, 6.80 roots/micro shoots) as well as root size (8.39, 5.73 cm) individually. Application: There are many opportunity of plant tissue culture which offer marvelous chances in plant propagation, plant development as well as creation of plants with necessary agronomical features. Finally often hardening plantlets were gradually adjusted to natural condition and acclimatized with 90% success. This established protocol could help plant cell biotechnology, horticulture, medical and industrial sector of the country.
Cestrum nocturnum, Micro Propagation, MS Salts Medium, Night Jasmine, Tissue Culture, Transplantable Plantlets
- Western Garden Book. 2007 Ed. Hortus Third Cornell University.
- Jawale C, Kirdak R, Dama L. Larvicidal activity of Cestrum nocturnumon Aedes aegypti. Bangladesh Journal of Pharmacol. 2010 Mar; 5(1):39–40.
- Patil CD, Patil SV, Salunke BK, Salunkhe RB, Bioefficacy of Plumbago zeylanica and Cestrum nocturnum plant extracts against Aedes aegypti and nontarget fish Poecilia reticulata. Parasitol Res. 2010 Nov; 108(5):1253–63.
- Perez-Saad H, Buznego MT. Behavioral and antiepileptic effects of acute administration of the extract of the plant Cestrum nocturnum Lin (lady of the night). Epilepsy Behav. 2008 Apr; 12(3):366–72.
- Huang LG, Zhang XC, Xiao H, Ye HY, Zeng J. Analgesic effect of Cestrum nocturnum L. extract on mice. Chin J Clin Rehab. 2006 Sep; 10(35):172–4.
- Catterjee SK, Bhattacharjee I, Chandra G. Bactericidal activities of some common herbs in India. Pharm Biol. 2007; 45(5):350–4.
- Zeng J, Li FZ, Ye HY. Study of the inhibitory effect of Cestrum nocturnum, L. n–butl alcohol extract on central nerve system. Journal of Gannan Medical University. 2003; 23(3):237–9.
- Mello JRB. Calcinosis-calcinogenic plants (Review). Toxicon. 2003; 41(1):1–12.
- Sahai M, Singh M, Singh AK, Hara N, Fujimoto Y. Cesternosides A and B, novel glucosides from the leaves of Cestrum nocturnum. J Chem Res Synop. 1994; 1:22–3.
- Mimaki Y, Ntonifor NN, Ngufor CA, Kimbi HK, Oben BO. Traditional use of mosquito repellent to protect human against mosquito and other insect bite sin rural community of Cameroon. East Afr Med J. 2006 Oct; 83(10):553–8.
- Jawale CS, Dama LB. Haematological changes in the fresh water fish, exposed to sub-lethal concentration of Piscicidal compounds. National Journal of Life Sciences. 2010; 7(1):82–4.
- Raj RSDP, Morais SM, Gopalakrishnan K. In vitro propagation and cell suspension culture of Callistemon citrinus L. Indian Journal of Science and Technology. 2010 Jan; 3(1):67–9. Doi no: 10.17485/ijst/2010/v3i1/29648.
- Karthikeyan S, Prasad R, Mahendran TS, Rajagopal K, Ravendran V. Direct regeneration and in vitro flowering of Scopariadulcis L. Indian Journal of Science and Technology. 2009 May; 2(5):55–7. Doi no: 10.17485/ijst/2009/v2i5/29467.
- Indira Iyer R, Jayaraman G, Ramesh A. In vitro responses and production of phytochemicals of potential medicinal value in Nutmeg, Myristica Fragrans Houtt. Indian Journal of Science and Technology. 2009 Apr; 2(4):65–70. Doi no: 10.17485/ijst/2009/v2i4/29434.
- Jawale CS, Vinchurkar AS, Dama LB, Pawar. Cestrum nocturnum (L) a prospective piscicide for control of predatory fish Channa punctatus (bloch.). Trends in Fisheries Research. 2012; 1(1):14–7.
- Backhouse NC, Delporte R, Salinas P, Pinto A, Aravena S, Cassels BK. Antiinflammatory and antipyretic activities of Cuscuta chilensis, Cestrum parqui and Psorela glandulosa. Int J Pharm.1996; 34(1):53–7.
- Hartmann HT, Kester DE, Davies FT, Geneve RL. Plant propagation, principles and practices. 7th ed. Prentice Hall; 2002.
- Jansky S, Stern KR, Bidlack JE. Introductory plant biology. Bon: The McGraw-Hill Companies Inc; 2004.
- Hirabayashi T, Moriguchi T, KozakiI, Ya-mamoto Y, Matsuzaki S. In vitro propagation of pears shoots tips. Bull Fruit Tree Res Stn Ser A.1987; 14:9–16.
- Druart Ph, Gruselle R. Plum (Prunus domestica). Biotechnology in Agriculture and Forestry, Trees II. Bajaj, Y.P.S. (Ed.) NewYork: Springer Berlin Heidelberg; 1986; 5:42–58.
- Singh S, Ray BK, Bhattacharryya S, Deka PC. In vitro propagation of C. reticulata Blanco and C. limon Burm. Hort Science. 1994; 29(3):214–6.
- Mohammed AA, Al–Younis MA. Fundamentals of plant physiology (In Arabic). Third part. College of Agriculture. Iraq: Baghdad Univ; 1991.
- Brookner N. Studies on the in vitro propagation of Mussaenda erythrophlla “Rosea”. (Trinidad and Tobago: St. Augustine; 1991. p. 181.
- Thanhran TK. Control morphogenesis in in vitro culture. Ann Rev Plant Physiol. 1981 Jun; 32:291–311.
- Roy PK, Mamun ANK, Ahmad G. In vitro plantlets regeneration of rose. Plant Tissue Culture. 2004 Dec; 14(2):149–54.
- Peak KY, Chandler SF, Thorpe TA. In vitro propagation of Chinese cabbage from seedling shoot tip. J Amer Soc Hort Sci. 1987; 112(5):841–5.
- George EF, Shermington PD. Plant propagation by tissue culture. Exegetics Ltd; Eversley England. 1984. p. 307–8.
- Saleh MS. Physiology of plant growth regulators. (In Arabic). Salahaddin. Univ Ministry of Higher Education and Scientific Research; IRAQ.1990 29. Gawel NJ, Robacker CD. Corly WL. In vitro propagation of Miscanthus sinesis. Hort. Science. 1990 Oct; 25(10):1291–3.
- Soni M, Thakur M, Modgil M. In vitro shoot multiplication of Merton I. 793, a clonal apple rootstock, suitable for replantation. Indian Journal of Science and Technology. 2011 Jul; 10(3):362–8.
- Reeves DW, Horton BD, Couvillon GA. Effect of media and media pHon in vitro propagation of Nemaguard peach rootstock. Hort Science. 1983 Dec; 21(4):353–7.
- Preece JE, Sutter EG. Acclimatization of micro propagation plants to greenhouse and field. Micropropagation Technology and Application; 1991. p. 71–93.
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