• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2009, Volume: 2, Issue: 12, Pages: 33-35

Original Article

An immunological analysis on sharing of antigens amongst gastro-intestinal nematodes using inhibition ELISA


Antigen sharing amongst gastro-intestinal nematodes viz., Haemonchus contortus, Oesophagostomum columbianum and Bunostomum trigonocephalum was evaluated by inhibition ELISA. Two antigens namely soluble extract antigen (SEA) and gut integral membrane antigen (GIMA) were prepared and hyper immune sera were raised in rabbits against SEA of three referral nematodes. Based on inhibition ELISA, it was observed that the SEA of H. contortus had 36.4 and 28.87% cross-reactivity with inhibited sera of B. trigonocephalum and O. columbianum respectively whereas, the GIMA showed 29.2 and 25.1% crossreactivity with B. trignocephalum and O. columbianum. The SEA of O. columbianum exhibited 34.3 and 22.0% and the GIMA revealed 27.3 and 22.8% cross-reactivity with B. trigonocephalum and H. contortus respectively. The SEA of B. trigonocephalum showed 24.8 and 34.2% and the GIMA exhibited 21.48 and 30.25% crossreactivity with O. columbianum and H. contortus respectively. Further, it was concluded that H. contortus showed a high degree of antigen sharing with B. trigonocephalum than O. columbianum.

Keywords: antigen sharing, nematodes, ELISA, Sheep. 


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