Antigenotoxicity of Tuberous Rhizomes of Kaempferia rotunda Linn. in Cyclophosphamide Induced Genotoxicity: In vitro and In vivo Study

Deepsika Chhetri; Y Jyothi; Lalhriatpuii; Sayed Sohaila; G Rachana

doi:10.17485/IJST/v16i47.2971

Article

Antigenotoxicity of Tuberous Rhizomes of Kaempferia rotunda Linn. in Cyclophosphamide Induced Genotoxicity: In vitro and In vivo Study

VIEWS 217
PDF 59

Indian Journal of Science and Technology

DOI: 10.17485/IJST/v16i47.2971

Year: 2023, Volume: 16, Issue: 47, Pages: 4577-4584

Original Article

Antigenotoxicity of Tuberous Rhizomes of Kaempferia rotunda Linn. in Cyclophosphamide Induced Genotoxicity: In vitro and In vivo Study

Deepsika Chhetri¹, Y Jyothi^1*, Lalhriatpuii¹, Sayed Sohaila¹, G Rachana¹

¹Department of Pharmacology, Krupanidhi College of Pharmacy, Bangalore, 560 035, Karnataka, India

*Corresponding Author
Email: [email protected]

Received Date:23 November 2023, Accepted Date:24 December 2023, Published Date:30 December 2023

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: To investigate the potential of a methanolic extract of Kaempferia rotunda Linn. rhizomes to mitigate the genotoxicity caused by cyclophosphamide. Methods: In this study, swiss albino mice of either sex were given a low dose of 200 mg/kg and a high dosage of 400 mg/kg of Kaempferia rotunda methanolic extract, followed by cyclophosphamide as a challenge, to test for antigenotoxic action. In vivo chromosomal aberration and micronuclei assays were conducted. Hydroxyl scavenging activity was conducted in vitro. Mention the statistical tool used. Findings: A significant decrease in PCE/NCE ratio was seen at doses of 200 mg/kg (1.763±0.445) and 400 mg/kg (1.241±0.871) of Kaempferia rotunda Linn. A significant reduction (18±2.54) in the chromosomal aberrations was found at 400 mg/kg of Kaempferia rotunda Linn. DPPH free radical scavenging activity of K. rotunda methanolic extract, demonstrated an increased IC50 value of 24.09μg/ml, in comparison to standard Ascorbic acid with IC50 of 8.17 μg/ml. The methanolic extract of K. rotunda exhibited a significant hydroxyl radical scavenging activity with an IC50 value of 10.22μg/ml when compared to standard Gallic acid with IC50 of 10.22μg/ml. Novelty: New medicines are being developed for the treatment of complicated diseases but these medicines are themselves associated with a number of side effects that range from minor to severe intensity. The medicines from nature on the other hand appear to be more effective than the synthetic counterpart. Our investigation confirms the genoprotective action of Kaempferia rotunda Linn.

Keywords: Genotoxicity, Chromosomal Aberration, Methanolic extract, Free radicals, Kaempferia rotunda linn

References

Elshamy AI, Mohamed TA, Essa AF, Abd-Elgawad AM, Alqahtani AS, Shahat AA, et al. Recent Advances in Kaempferia Phytochemistry and Biological Activity: A Comprehensive Review. Nutrients. 2019;11(10):1–33. Available from: https://doi.org/10.3390/nu11102396
Madić V, Stojanović-Radić Z, Jušković M, Jugović D, Popović AŽ, Vasiljević P. Genotoxic and antigenotoxic potential of herbal mixture and five medicinal plants used in ethnopharmacology. South African Journal of Botany. 2019;125:290–297. Available from: https://doi.org/10.1016/j.sajb.2019.07.043
Stein J, Jorge BC, Reis ACC, Radai JAS, Moreira SDS, Fraga TL, et al. Evaluation of the safety of ethanolic extract from Piper amalago L. (Piperaceae) leaves in vivo: Subacute toxicity and genotoxicity studies. Regulatory Toxicology and Pharmacology. 2022;129:105118. Available from: https://doi.org/10.1016/j.yrtph.2022.105118
Liu YQ, Lu XX, Hu KX, Peng XBB, Jiang Y, Han LM, et al. Dynamic observation of bone marrow suppression and chromosomal aberrations in patients with acute colchicine poisoning. World Journal of Emergency Medicine. 2022;13(2):130–134. Available from: https://doi.org/10.5847%2Fwjem.j.1920-8642.2022.030
Kocaman AY, Yakar B. Genotoxicity of a synthetic plant growth regulator, Forchlorfenuron (CPPU), on human lymphocytes using chromosome aberration assay. Caryologia. 2023;76(1):47–56. Available from: https://doi.org/10.36253/caryologia-2006
Akwanjoh SR, Abdoul N, Akacha EL, Ingrid DT. Cytogenotoxicity of the Aqueous Extract of Bitter Kola (Garcinia kola: Clusiaceae) Using Allium cepa Assay. American Journal of BioScience. 2022;10(6):195–200. Available from: https://www.sciencepublishinggroup.com/article/10.11648.j.ajbio.20221006.13
Saeed MK, Zahra N, Abidi SHI, Syed Q. Phytochemical screening and DPPH free radical scavenging activity of Aloe vera (Aloe barbadensis Miller) powder. International Journal of Food Science and Agriculture. 2022;6(3):301–308. Available from: https://wap.hillpublisher.com/UpFile/202208/20220826142703.pdf
Fan L, Lin L, Zhang Y, Li S, Tang Z. Component characteristics and reactive oxygen species scavenging activity of anthocyanins from fruits of Lonicera caerulea L. Food Chemistry. 2023;403:134391. Available from: https://doi.org/10.1016/j.foodchem.2022.134391
Vukić MD, Vuković NL, Mladenović M, Tomašević N, Matić S, Stanić S, et al. Chemical Composition of Various Nepeta cataria Plant Organs’ Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations. Plants. 2022;11(16):1–31. Available from: https://doi.org/10.3390/plants11162114
Malik S, Kaur K, Prasad S, Jha NK, Kumar V. A perspective review on medicinal plant resources for their antimutagenic potentials. Environmental Science and Pollution Research . 2022;29:62014–62029. Available from: https://doi.org/10.1007/s11356-021-16057-w

Copyright

© 2023 Chhetri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)