Assessment on the Physicochemical and Phytochemical Properties, Nutritional and Heavy Metal Contents, and Antioxidant Activities of Hylocereus polyrhizus Peel from Northern Philippines

Ma  Danica Ines Ramil; Angelica May DC  Mendoza; Rhian Jaymar D  Ramil

doi:10.17485/IJST/v14i14.2286

Article

Assessment on the Physicochemical and Phytochemical Properties, Nutritional and Heavy Metal Contents, and Antioxidant Activities of Hylocereus polyrhizus Peel from Northern Philippines

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PDF 433

Indian Journal of Science and Technology

DOI: 10.17485/IJST/v14i14.2286

Year: 2021, Volume: 14, Issue: 14, Pages: 1097-1104

Original Article

Assessment on the Physicochemical and Phytochemical Properties, Nutritional and Heavy Metal Contents, and Antioxidant Activities of Hylocereus polyrhizus Peel from Northern Philippines

Ma. Danica Ines Ramil¹, Angelica May DC. Mendoza¹,Rhian Jaymar D. Ramil^1*

¹Assistant Professors, Pharmacy Department, Mariano Marcos State University, City of Batac, Ilocos Norte, 2906, Philippines. Tel.: (+63) 927 733 9569

*Corresponding Author
Tel: (+63) 927 733 9569
Email: [email protected]

Received Date:22 December 2020, Accepted Date:31 March 2021, Published Date:26 April 2021

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background/Objectives: In the Philippines, Hylocereus polyrhizus is gaining upsurge interest due to its potential health benefits. Aside from the edible fruit pulp, various products from its peel are being developed, and claimed as health boosters. Thus, this study was conducted to assess the physicochemical characteristics, phytochemical constituents, nutritional and heavy metal contents and antioxidant activity of the peel of H. polyrhizus. Methods/Statistical analysis: Physicochemical and phytochemical screening were conducted following established protocols. Nutritional value and trace metals were measured with food analytical standard methods. Antioxidant activity was estimated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing capacity (FRC) assays. Statistical analyses were carried out for DPPH and FRC assays using Graph-Pad Prism. Findings: Results showed that H. polyrhizus peel contains alkaloids, coumarins, flavonoids, phenols, and triterpenoids. Trace amounts of arsenic (0.022 mg/kg), lead (0.887 mg/kg), cadmium (0.068 mg/kg) and chromium (0.225 mg/kg) were detected which were all within the acceptable limit set by the WHO for herbal materials. H. polyrhizus peel contained 6.72% moisture, 14.7% ash, 0.526% total fat, 0.226% saturated fat, 3.73% total sugar, 4.28% crude protein, 73.8% total carbohydrate, and 63.2% total dietary fiber. Other nutrients detected are macronutrients such as K > Ca > Mg > Na, micronutrients Mn > Fe, and Vitamin C. Moreover, it exhibited remarkable antioxidant activity in terms of FRC assay and DPPH assay (IC50 = 986.8mg/mL). The present study results showed that H. polyrhizus peel could be used as a potentially rich source of nutrients and natural antioxidants. Thus, it can be developed as an ingredient for food and health products. Novelty/Applications: The preliminary data obtained provide information that could support the development of products from dragon fruit peel. The conversion of these wastes into utilizable natural health products would help in reducing environmental problems Ma. Danica Ines Ramil et al. / Indian Journal of Science and Technology 2021;14(14):1097–1104 associated with processing waste disposal.

Keywords: Antioxidant; Dragon Fruit Peel; Hylocereus polyrhizus; Phytochemical Screening; Nutritional Content; Heavy Metals

References

Pascua LT, Pascua ME, Gabriel M. Dragon fruit production and marketing in the Philippines: Its status, constraints and prospects. In: YLJ, PCL, PHH., eds. Improving Pitaya Production and Marketing . (pp. 47-63) 2012.
Perween T, Mandal K, Hasan M. Dragon fruit: An exotic super future fruit of India. J Pharmacogn Phytochem. 2018;7(2):1022–1028. Available from: http://www.phytojournal.com/archives/2018/vol7issue2/PartO/7-1-435-453.pdf
Tagay AA. Supply chain analysis of dragon fruit in Ilocos Norte, Philippines. International Journal of Engineering Researches and Management Studies. 2017;4(4):11–27. Available from: https://www.ijerms.com/DOC/Isuespdf/Archive-2017/April-2017/3.pdf
Pathak PD, Mandavgane SA, Kulkarni BD. Fruit peel waste as a novel low-cost bio adsorbent. Reviews in Chemical Engineering. 2015;31(4):361–381. Available from: https://dx.doi.org/10.1515/revce-2014-0041
Khattak KF, Taj-Ur-Rahman. Effect of geographical distributions on the nutrient composition, phytochemical profile and antioxidant activity of Morus nigra. Pak J Pharm Sci. 2015;28(5):1671–1679. Available from: http://www.pjps.pk/wp-content/uploads/pdfs/28/5/Paper-16.pdf
WHO. Quality control methods for herbal materials. World Health Organization. 2011.
Luo H, Cai Y, Peng Z, Liu T, Yang S. Chemical composition and in vitroevaluation of the cytotoxic and antioxidant activities of supercritical carbon dioxide extracts of pitaya (dragon fruit) peel. Chemistry Central Journal. 2014;8(1):1–7. Available from: https://dx.doi.org/10.1186/1752-153x-8-1
AOAC (Association of Official Analytical Chemistry) In: Official methods of analysis (19). Gaithersburg. 2012.
Karagöz A, Artun FT, Özcan G, Melikoğlu G, Anıl S, Kültür Ş, et al. In vitroevaluation of antioxidant activity of some plant methanol extracts. Biotechnology & Biotechnological Equipment. 2015;29(6):1184–1189. Available from: https://dx.doi.org/10.1080/13102818.2015.1080600
Benzie IFF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry. 1996;239(1):70–76. Available from: https://dx.doi.org/10.1006/abio.1996.0292
Lim C, Lim SL. Ferric Reducing Capacity Versus Ferric Reducing Antioxidant Power for Measuring Total Antioxidant Capacity. Lab Med. 2013;44(1):51–56. doi: 10.1309/LM93W7KTFNPZIXRR
Amudha M, Rani S. Evaluation of In Vitro Antioxidant Potential of Cordia retusa. Indian Journal of Pharmaceutical Sciences. 2016;78(1):80–86. Available from: https://dx.doi.org/10.4103/0250-474x.180253
Hell K, Mutegi C. Aflatoxin control and prevention strategies in key crops of Sub-Saharan Africa. Afr J Microbiol Res. 2011;5(5):459–66. Available from: http://crsps.net/wp-content/downloads/Peanut/Inventoried%208.8/7-2011-4-142.pdf
Vaikosen EN, Alade GO. Evaluation of pharmacognostical parameters and heavy metals in some locally manufactured herbal drugs. J Chem Pharm Res. 2011;3(2):88–97.
Mukherjee PK. Qualitative Analysis for Evaluation of Herbal Drugs. In: Evaluating Natural Products and Traditional Medicine. (pp. 79-149) Elsevier. 2019.
Thawabteh A, Juma S, Bader M, Karaman D, Scrano L, Bufo S, et al. The Biological Activity of Natural Alkaloids against Herbivores, Cancerous Cells and Pathogens. Toxins. 2019;11(11):1–28. Available from: https://dx.doi.org/10.3390/toxins11110656
Tenore GC, Novellino E, Basile A. Nutraceutical potential and antioxidant benefits of red pitaya (Hylocereus polyrhizus) extracts. Journal of Functional Foods. 2012;4(1):129–136. Available from: https://dx.doi.org/10.1016/j.jff.2011.09.003
Boo YC. Coumaric acid as an active ingredient in cosmetics: A review focusing on its antimelanogenic effects. Antioxidants. 2019;8(8):1–16. Available from: https://doi.org/10.3390/antiox8080275
Maleki SJ, Crespo JF, Cabanillas B. Anti-inflammatory effects of flavonoids. Food Chemistry. 2019;30(299). Available from: https://dx.doi.org/10.1016/j.foodchem.2019.125124
Souza POd, Bianchi SE, Figueiró F, Heimfarth L, Moresco KS, Gonçalves RM, et al. Anticancer activity of flavonoids isolated from Achyrocline satureioides in gliomas cell lines. Toxicology in Vitro. 2018;51:23–33. Available from: https://dx.doi.org/10.1016/j.tiv.2018.04.013
Ellwood L, Torun G, Bahar Z, Fernandez R. Effects of flavonoid rich fruits on hypertension in adults: A systematic review. JBI Database of System Rev Implement Rep. 2019;17(10):2075–2105. doi: 10.11124/JBISRIR-D-19-00050
Murata RM, Pasetto S, Pardi V. The anti-HIV activity of flavonoids: Quercetin and Pinocembrin. Planta Medica. 2014;80(10):PE9. Available from: https://dx.doi.org/10.1055/s-0034-1382573
Haas ICdS, Marmitt DJ, Fedrigo IMT, Goettert MI, Bordignon-Luiz MT. Evaluation of antiproliferative and anti-inflammatory effects of non-pomace sediment of red grape juices (Vitis labrusca L.) in healthy and cancer cells after in vitro gastrointestinal simulation. PharmaNutrition. 2020;13. Available from: https://dx.doi.org/10.1016/j.phanu.2020.100204
Kassegn HH. Determination of proximate composition and bioactive compounds of the Abyssinian purple wheat. Cogent Food & Agriculture. 2018;4(1):1–9. Available from: https://dx.doi.org/10.1080/23311932.2017.1421415
Jamilah B, Shu CE, Kharidah M, Dzulkifly MA, Noranizan A. Physico-chemical characteristics of red pitaya (Hylocereus polyrhizus) International Food Research Journal. 18:279–286. Available from: http://psasir.upm.edu.my/id/eprint/16208/1/16208.pdf
Caprita A, Caprita R, Simulescu V, Drehe R. The effect of temperature on soluble dietary fiber fraction in cereals. J Agroaliment Processes Technol. 2011;17(3):214–231.
Soliman GA. Dietary Fiber, Atherosclerosis, and Cardiovascular Disease. Nutrients. 2019;11(5):1–11. Available from: https://dx.doi.org/10.3390/nu11051155
Stamler J, Chan Q, Daviglus ML, Dyer AR, Horn LV, Garside DB, et al. Relation of Dietary Sodium (Salt) to Blood Pressure and Its Possible Modulation by Other Dietary Factors. Hypertension. 2018;71(4):631–637. Available from: https://dx.doi.org/10.1161/hypertensionaha.117.09928
Muhammad I, Ashiru S, Ibrahim I, Kanoma I, Sani I, Garba S. Effect of ripening stage on vitamin C content in selected fruits. Int J Agric For Fish. 2014;2(3):60–65.
Dong OM. Excessive dietary sodium intake and elevated blood pressure: a review of current prevention and management strategies and the emerging role of pharmaconutrigenetics. BMJ Nutr Prev Health. 2018;1(1):7–16.
Rahman MM, Khan MMR, Hosain MM. Analysis of vitamin C (ascorbic acid) contents in various fruits and vegetables by UV-spectrophotometry. Bangladesh Journal of Scientific and Industrial Research. 2007;42(4):417–441.
Grosso G, Bei R, Mistretta A, Marventano S, Calabrese G, Masuelli L. Effects of vitamin C on health: A review of evidence. Front Biosci. 2013;18(3):1017–1046.
Choo WS, WKY. Antioxidant properties of two species of Hylocereus fruits. Adv Appl Sci Res. 2011;2(3):418–443.
Tsai Y, Lin CG, Chen WL, Huang YC, Chen CY, Huang KF. Evaluation of the antioxidant and wound-healing properties of extracts from different parts of Hylocereus polyrhizus. Agronomy. 2019;9(1):1–10. Available from: https://doi.org/10.3390/agronomy9010027
Nurliyana R, Zahir IS, Suleiman KM, Aisyah MR, KKR. Antioxidant study of pulps and peels of dragon fruits: A comparative study. Int Food Res J. 2010;17(2):367–375.
Hendra R, Masdeatresa L, Abdulah R, Haryani Y. Red dragon peel (Hylocereus polyrhizus) as antioxidant source. AIP Conference Proceedings. 2020;2243:10–14. doi: 10.1063/5.0001391
Zhang Q, Shao J, Zhao T, He L, Ma H, Jing L. The Role of C-8 OH on the Antioxidant Activity of Norwogonin and Isowogonin. Natural Product Communications. 2020;15(5):1–7. Available from: https://dx.doi.org/10.1177/1934578x20924887
Amadou I, Le GW, Shi YH, Jin S. Reducing, Radical Scavenging, and Chelation Properties of Fermented Soy Protein Meal Hydrolysate byLactobacillus plantarumLP6. International Journal of Food Properties. 2011;14(3):654–665. Available from: https://dx.doi.org/10.1080/10942910903312502
Gülçin I. Fe3+-Fe2+ transformation method: An important antioxidant assay. In: AD., ed. Advanced Protocols in Oxidative Stress III Methods in Molecular Biology . (pp. 1-477) Humana Press. 2014. 10.1007/978-1-4939-1441-8_17

Copyright

© 2021 Ma. Danica Ines Ramil et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)