Indian Journal of Science and Technology

Abstract

Objectives: Study of biphenyl degrading bacteria from probable PCB contaminated soil at Anjaneya Mill Locality, Davangere, Karnataka, India. Methods: A novel biphenyl degrading bacterial isolate GS-008 was isolated from transformer oil contaminated soil. During Biphenyl catabolic pathway, yellow metabolite developed on addition of 2,3- dihydroxybiphenyl dioxygenase substrate and therefore enriched colonies appeared. Further, the isolate GS 008 was characterized 16S rDNA typing identified as Stenotrophomonas sp. Findings: GC analysis revealed that the significant Biphenyl degrading activity of GS-008. The degradation of biphenyl was detected with the productionof 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoicacid (HOPDA) and their extent of production was determined using range of time points. Novelty: GS-008 bacterial isolateis one of the promising isolates to degrade the 2,3- dihydroxybiphenyl various PCB contaminated sites.
Keywords: 3 biphenyldioxygenase, 4 dienoic acid, 2-Hydroxy 6-oxo 6-phenyl hexa 2, Stenotrophomonas GS-008, Substrate biphenyl;2