Indian Journal of Science and Technology

Abstract

Nano-silver particles are the integral aspect in functional nanotechnology filed. These nanoparticles have antibacterial and antifungal characteristics. Combination therapy is one of the new and specific methods for therapy of diseases in medicine and pharmacology fields. Interaction of alcoholic eucalyptus and nano-silver could be not performed in many investigations. For this reason, in this investigation, synergic effect of alcoholic eucalyptus and nano-silver in growth inhibition of Aspergilus niger were assayed A.niger were cultured in PDA medium. These fungi were treated with nanosilver and nano-silver -Eucalyptus ethanol extraction mixture in 12.5 and 50-ppm concentration. Then, Morphological changes and colony number in treatment and control groups were compared. Results of these investigations were shown that growth rate of A.niger decreased significantly (P<.01) 8 day after treatment. In addition, growth and colony number of A.niger decreased significantly (P<0.01) in 12.5ppm concentration of nano-silver -Eucalyptus 24 day after treatment. These found could be suggested the therapy of human fungal diseases in medicine will be performed with mixture of nano-silver and eucalyptus ethanol extract. This nanomaterial could be used as antifungal agents. Specific investigations are necessary to improved that this combination has not any side effect in human. This nanomaterial could be suggested as a minor antifungal drug.
Keywords: Nano-silver , Nanobiotechnology, Eucalyptus ethanol extract, Aspergilus niger.