• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2023, Volume: 16, Issue: 41, Pages: 3627-3639

Original Article

Eclipta alba, An Antioxidant-Rich Memory Enhancer: In-Silicoand In-Vivo Evidence with Specific Insight into Alzheimer’s Disease

Received Date:20 April 2023, Accepted Date:28 September 2023, Published Date:07 November 2023


Objectives: Eclipta alba (EA), a potent anti-aging drug with unidentified secondary metabolites, is a widely recommended nerve tonic that is used to treat dementia. This study aimed to identify the secondary metabolite profile of EA, followed by their interaction with Alzheimer’s disease (AD) related proteins. Therefore, the present study attempted to screen the active constituents of the aqueous extract of EA (aqEA) using liquid chromatography-mass spectrometry (LC-MS) and to identify its effect on spatial learning and memory also understanding its toxicity in in-vivo. Methodology: Initially, Autodocking analysis was performed to analyze the molecular interactions of secondary metabolites with AD-related proteins followed by in-vivo investigations using albino Wistar rats, where 150 mg/kg of aqEA was given orally for 28 days and memory was analyzed using 8-arm radial maze(RAM). Blood parameters were screened for toxicity related parameters using an auto analyzer. Findings: LC-MS showed a significant proportion of Lilaline, Laccarin, Coriandrone E, Quercetin, Catechin, Wedelolactone, and Luteolin. Autodocking showed a good binding affinity for each phytochemical, with coriandrone E being the most effective. Further aqEA elicited no significant toxic effects in in-vivo and interestingly improved memory performance in the RAM. Novelty: This study identified unique pharmacologically active metabolites in aqEA that interacted well with AD related proteins with higher affinity thereby providing valuable clue on aqEA as a suitable drug against AD pathology. Interestingly, aqEA observed to influence cholinergic neurons by regulating acetylcholine esterase activity which could replace wide commercial inhibitors available in the market. Thereby the current study paves way for more unidentified pathways that could be addressed by the treatment of aqEA through its active secondary metabolites.

Keywords:  Dementia, Alzheimer's Disease, Aging, Eclipta alba, Neuroprotection


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© 2023 Abubakkar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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