• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2023, Volume: 16, Issue: 47, Pages: 4594-4604

Original Article

Evaluation of Haemopocitic Activity of Crotalaria juncea Seed Powder Suspension in Phenylhydrazine Induced Haemolytic Anaemia in Rats

Received Date:24 November 2023, Accepted Date:23 December 2023, Published Date:30 December 2023


Objectives: To investigate the anti-anaemic activity of Crotalaria juncea seed powder suspension against phenylhydrazine induced haemolytic anaemia in rats. Methods: The seeds of Crotalaria juncea was subjected to preliminary phytochemical analysis. Anemia was induced by administering phenylhydrazine (40 mg/kg, i.p.) for 2 consecutive days. Antianemic activity of Crotalaria juncea was investigated at the dose of 200 mg/kg and 400 mg/kg, once daily for 21 days by estimating blood parameters and pathological changes in kidney, liver, heart, spleen, and heart. Findings: Crotalaria juncea was showed the presence of alkaloids, carbohydrates, tannins, flavonoids, and phenolic compounds. Iron content was found to be 5 mg/100 ml in Crotalaria juncea. Anemia induction by phenylhydrazine injections to rats caused significant decrease in red blood cells, hemoglobin, and packed cell volume. These decreased levels of RBCs, hemoglobin and PCV in blood was significantly improved by the treatment with Crotalaria juncea (p<0.05). A significant normalized clotting time equivalent to normal control groups was observed in group treated with C. juncea. Furthermore, Crotalaria juncea restored pathological changes in kidney, liver, and heart tissues near to normal. Novelty: The C. juncea can be used as anti-anemic treatment with fewer side effects.

Keywords: Anaemia, Crotalaria juncea, Phenylhydrazine, Haemoglobin, Anti-anaemic activity


  1. Turner J, Parsi M, Badireddy M, . Anemia. Treasure Island, Finland. StatPearls Publishing. 2022. Available from: https://www.ncbi.nlm.nih.gov/books/NBK499994/
  2. Safiri S, Kolahi AAA, Noori M, Nejadghaderi SA, Karamzad N, Bragazzi NL, et al. Burden of anemia and its underlying causes in 204 countries and territories, 1990–2019: results from the Global Burden of Disease Study 2019. Journal of Hematology & Oncology. 2021;14(1):1–16. Available from: https://doi.org/10.1186/s13045-021-01202-2
  3. Maladkar M, Sankar S, Yadav A. A novel approach for iron deficiency anaemia with liposomal iron: concept to clinic. Journal of Biosciences and Medicines. 2020;8(9):27–41. Available from: https://doi.org/10.4236/jbm.2020.89003
  4. Sheth PA, Pawar AT, Mote CS, More C. Antianemic activity of polyherbal formulation, Raktavardhak Kadha, against phenylhydrazine-induced anemia in rats. Journal of Ayurveda and Integrative Medicine. 2021;12(2):340–345. Available from: https://doi.org/10.1016/j.jaim.2021.02.009
  5. Irinmwinuwa EO, Adolphus MC, Opute RE, Oyate GB, Chinedu JO, Afonne OJ, et al. Review of herbal plant associated with anti-anemic property and mechanism of action. International Journal of Science and Research Archive. 2023;10(2):589–601. Available from: https://doi.org/10.30574/ijsra.2023.8.2.0305
  6. Patil RR, Navghare AA. Medicinal plants for treatment of anemia: a brief review. World Journal of Pharmaceutical Research. 2019;8(5):701–717. Available from: https://wjpr.s3.ap-south-1.amazonaws.com/article_issue/1554109459.pdf
  7. Kumar AR, Gunalan G, Firdouse KHH, Archana A. Pre-clinical studies of Siddha formulations advocated for anaemia: A systematic review and meta-analysis. Journal of Applied Pharmaceutical Science. 2023;13(8):185–203. Available from: http://dx.doi.org/10.7324/JAPS.2023.126389
  8. Zarei MH, Lorigooini Z, Khoei HA, Bijad E. Acute oral toxicity assessment of galbanic acid in albino rat according to OECD 425 TG. Toxicology Reports. 2023;11:111–115. Available from: https://doi.org/10.1016/j.toxrep.2023.07.001
  9. Salem SA, Gad AM, Kamal AA. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. International Journal of Food Science and Biotechnology. 2021;6(2):21–29. Available from: https://doi.org/10.11648/j.ijfsb.20210602.11
  10. Irinmwinuwa EO, Adolphus MC, Opute RE, Oyate GB, Chinedu JO, Afonne OJ, et al. Review of herbal plant associated with anti-anemic property and mechanism of action. International Journal of Science and Research Archive. 2023;10(2):589–601. Available from: https://doi.org/10.30574/ijsra.2023.8.2.0305
  11. Odiegwu CN, Chianella I, Azubike NC, Odiegwu UO, Ogbuowelu OS. Liver Function Tests Values in Albino Wistar Rats Administered with Isolated Nigeria Achatina achatina Snail Lectin. GSC Biological and Pharmaceutical Sciences. 2021;15(2):92–102. Available from: https://doi.org/10.30574/gscbps.2021.15.2.0115
  12. Ousaaid D, Ghouizi AE, Laaroussi H, Bakour M, Mechchate H, Es-Safi I, et al. Anti-Anemic Effect of Antioxidant-Rich Apple Vinegar against Phenylhydrazine-Induced Hemolytic Anemia in Rats. Life. 2022;12(2):1–14. Available from: https://doi.org/10.3390/life12020239
  13. Akara EU, Emmanuel O, Ude VC, Uche-Ikonne C, Eke G, Ugbogu EA. Ocimum gratissimum leaf extract ameliorates phenylhydrazine-induced anaemia and toxicity in Wistar rats. Drug metabolism and personalized therapy. 2021;36(4):311–320. Available from: https://doi.org/10.1515/dmpt-2020-0185
  14. Nalimu F, Oloro J, Peter EL, Ogwang PE. Acute and sub-acute oral toxicity of aqueous whole leaf and green rind extracts of Aloe vera in Wistar rats. BMC Complementary Medicine and Therapies. 2022;22(1):1–14. Available from: https://doi.org/10.1186/s12906-021-03470-4
  15. Fagbohoun L, Nonvidé GC, Orou AS, Houngbèmè A, Sakirigui A, Gunin F, et al. Anti-Anaemic Activity and Potential Toxicity of Extracts of Four Tinctorial Plants Used in the Treatment of Anemia in Benin: Gossypium barbadense, Sorghum bicolor, Hibiscus sabdariffa and Justicia secunda. American Journal of Plant Sciences. 2022;13(12):1460–1477. Available from: https://doi.org/10.4236/ajps.2022.1312100
  16. Obayuwana E, Imafidon EO, Odiase DE, Alih OJ, Nweke SM, Enoghase RJ, et al. Effects of Aqueous Extract of Moringa oleifera on Phenylhydrazine-induced Liver Toxicity in Wistar Rats. Journal of Applied Sciences and Environmental Management. 2022;26(5):949–954. Available from: https://doi.org/10.4314/jasem.v26i5.23


© 2023 Lalhriatpuii et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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