Indian Journal of Science and Technology
Year: 2018, Volume: 11, Issue: 13, Pages: 1-7
Oscar Eduardo Rodriguez Aguirre1*and Ruben Dario Torrenegra Guerrero2
1 Engineering Faculty, Environmental Engineering Program, Universidad El Bosque (Bogota, Colombia); [email protected]
2 Faculty of Sciences, Universidad de Ciencias Aplicadas y Ambientales (University of Applied and Environmental Sciences, U.D.C.A.), Bogota, Colombia; [email protected]
*Author for correspondence
Oscar Eduardo Rodriguez Aguirre,
Engineering Faculty, Environmental Engineering Program, Universidad El Bosque (Bogota, Colombia); [email protected]
Objective: To purify and identify secondary metabolites of Diplostephium phylicoides and determine the antioxidant capacity through the DPPH* and ABTS*+ techniques. Methods: Extracts were obtained by soxhlet of the different organs with solvents of increasing polarity and by means of column chromatography, triterpenes and flavonoids were purify, which were identify by spectroscopic techniques. Findings: It is purified and identified in leaves: Urs-12-ene-3, 28-diol (Uvaol), 5, 4’-dihydroxy-7-methoxy-flavone (Genkwanin) and 5, 6,4’-trihydroxy-7-methoxy-flavone (Sorbifolin). In the stems were found Bauer-7-en-3b-yl acetate (Bauerenol acetate) and in the flowers, friedelan-3-one (Friedelin) and 3, 3’, 4’, 5, 7-pentahydroxyflavone (Quercetin). The anti-oxidant activity was determined by the DPPH* and ABTS*+ free radical assay, with the polar extracts showing a greater activity. The ethanol extract showed an IC50 of 13.80 mg/L MeOH, with the DPPH* assay and 10.14 mg/L MeOH, with the ABTS*+ assay. The extract of ethyl acetate showed an IC50 of 28.32 mg/L MeOH, with the DPPH*assay and 10.86 mg/L MeOH, with the ABTS*+ assay method. Application: The species Diplostephium phylicoides possesses a high antioxidant activity in the polar fractions, which present the flavonoids, positioning the species with a high medicinal potential.
Keywords: ABTS*+, DPPH* , Diplostephium phylicoides (H.B.K.) Wedd., Flavonoids, Terpenes
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