Genetic diversity in commercial varieties of chilli as revealed by RAPD method

H  K  Makari; H  S  Ravikumar Patil; M  Abhilash  and H  D  Mohan Kumar

doi:10.17485/ijst/2009/v2i4.18

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Genetic diversity in commercial varieties of chilli as revealed by RAPD method

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2009/v2i4.18

Year: 2009, Volume: 2, Issue: 4, Pages: 91-94

Original Article

Genetic diversity in commercial varieties of chilli as revealed by RAPD method

H. K. Makari¹ , H. S. Ravikumar Patil¹ , M. Abhilash² and H. D. Mohan Kumar³

¹Dept. of Biotech., G.M. Instt. of Technol., Davangere ,
²The Oxford College of Engg., Bangalore , Karnataka, India
³Dept. of Plant Breeding & Genetics, Agri. Res. Station, Univ. Agri. Sci., Devihosur, Haveri, Karnataka,India

*Author for the correspondence:
H. D. Mohan Kumar
Dept. of Plant Breeding & Genetics, Agri. Res. Station, Univ. Agri. Sci., Devihosur,
Haveri, Karnataka,India
E-mail: [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The study aimed at investigating the genetic diversity of ten different commercial varieties of chilli using random amplified polymorphic DNA (RAPD) analysis. The DNA extracted by CTAB method from the leaf sample was amplified by successive PCR cycles. Using 12 different primers, the amplified DNA solution was subjected to agarose gel electrophoresis. Around 500 different bands were observed under UV light. Seven were polymorphic for a specific primer and can be used as differential markers. This effort can serve determining genetic diversity among ten commercial varieties of chilli in India.

Keywords: Chilli, RAPD, molecular markers, RFLP