Indian Journal of Science and Technology

Abstract

Background: We investigated the possibility of using ion-beam processing to create biomatrix which is used to produce monolayer for cell cultures on a photo glass. Methods: Experimental setup with the Kaufman type ion source which produces beams of inert gases and nitrogen in diameter of 100 mm, with an energy in the range of 0.1-1.7 keV, ion current density to 500 mA/cm2 has been used. Results: Hepatocytes of mice, which deposited on the glass surface after ion-beam treatment better adsorbed (2.8 ± 0.2 times higher) than the control (untreated) and exhibited the formation of cell aggregates. The number of human fibroblasts, which adsorbed on the matrix, in (1.5 ± 0.5) times exceeds number of cells than on a smooth glass. It has been established that fibroblasts have a normal configuration for this type of cell onto modified glass. It has also been shown that cells “Candida albicans” are 5 times more adsorbed onto the modified glass than on the control glass. Novelty: Extract of the hepatocytes of mice, which is incubated on a modified glass, contains protein fractions with molecular weights of 30, 43 and 120 kDa which are not present on the control sample. 

Keywords: Adhesion, Cell Culture, Fibroblasts, Glass, Hepatocytes, Ion-Beam Processing, Surface Modification