• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology

Article

Indian Journal of Science and Technology

Year: 2009, Volume: 2, Issue: 11, Pages: 55-58

Original Article

Molecular characterization of vembur sheep (Ovis aries) of south India based on microsatellites

Abstract

The Vembur sheep is a mutton breed, adapted to semi arid habitat and distributed in and around Thoothukudi, Virudhunagar and Tirunelveli districts of Tamilnadu, India. Microsatellite characterization yielded a total of 147 alleles in the sampled population for 25 marker loci under investigation, with a mean of 5.88 ± 0.29 alleles per locus. Number of alleles observed on each locus varied between two and nine. The effective number of alleles observed with a mean of 4.0950 ± 0.23 alleles per locus. The mean PIC value was 0.6905 ± 0.02. The frequency of majority of the loci returned highly significant chi-square values suggesting departure from HWE. The observed heterozygosity (Ho) values varied between 0.1333 and 1.0000, while the expected (He) values ranged from 0.4847 to 0.8537. The mean values were 0.5202 ± 0.04 and 0.7339 ± 0.02 respectively. FIS estimates were negative for three loci indicating more heterozygous nature of the population. The mean value observed was 0.2954 ± 0.31.

Keywords: Vembur sheep, microsatellites, PIC, FIS, India.

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