Indian Journal of Science and Technology

Abstract

Objective: Present study was carried out to establish the biocontrol potentiality of nematode extract on Lycopesicon esculentum (Tomato) Pusa Ruby variety infected with Meloidogyne incognita (Kofoid & White) Chitwood nematode. Methods/Statistical analysis: We examine in vitro test, phytotoxicity test, in vivo test, in vivo glasshouse bioassay test, PAL (Phenylalanine ammonia lyase) extraction test to identify the effect of nematode extract on tomato plant. One way analysis of variance, ANOVA test is performed in this experiment. Findings: The result of in vitro laboratory bioassay showed that application of nematode extract safe for second-stage juveniles (J₂) of M. incognita. The result of in vivo test revealed that nematode extract increased growth of inoculated plants in terms of shoot length, shoot weight and root length as compared with inoculated untreated plants. Application of nematode extract showed reduction in root gall number and number of nematode eggs in inoculated roots. PAL (Phenylalanine ammonia lyase) activity increased in roots of nematode extract treated plants. Root protein content was greater in inoculated untreated plants compared to treated groups. Application: This is the first study to control plant parasitic nematode M. incognita with nematode extract. In the future it will minimize the global crop loss.

Keywords: Biocontrol, Nematode, PAL activity, Root gall