Indian Journal of Science and Technology
Year: 2018, Volume: 11, Issue: 46, Pages: 1-9
Jorge Noriega M1*, Darwin Hernández H2 , Moris Bustamante Yánez1 , Luz Ángela Álvarez3 , Manuel Fernando Ariza B4 , Oscar Vergara G1
1 Universidad de Córdoba, Facultad de Medicina Veterinaria y Zootecnia, Grupo de Investigación en Producción Animal Tropical, Montería, Colombia; [email protected], [email protected] unicordoba.edu.co, [email protected]
2 Universidad de Sucre, Facultad de Ciencias Agropecuarias, Grupo de Investigación en Reproducción y Mejoramiento Genético Animal, Sincelejo, Colombia; [email protected]
3 Universidad Nacional de Colombia, sede Palmira; [email protected]
4 Universidad Nacional de Colombia, sede Bogotá, facultad de Ciencias; [email protected]
*Author for correspondence
Jorge Noriega M,
Universidad de Córdoba, Facultad de Medicina Veterinaria y Zootecnia, Grupo de Investigación en Producción Animal Tropical, Montería, Colombia; [email protected]
Objective: The study was to characterize the genetic polymorphism of 23 SNPs located in candidate growth genes in Colombian Creole hair (OPC). Materials and Methods: We used 106 individuals belonging to two OPC subpopulations located in the departments of Cesar (CS) and Córdoba (CB), these were genotyped using the OvineSNP50 Bead Chip of Illumina ®, were calculated frequencies genotypic, allelic, the Hetero zygosity observed (Ho) and expected (he), the Fixation Index (F), the Hardy-Weinberg equilibrium deviations (EHW) and a molecular variance analysis to estimate the values of FST, FIS and FIT. Findings: The minimum allelic frequency (MAF) varied from 0.042 to 0.491 in the entire OPC population, while in the CS subpopulation the values varied from 0.037 to 0.481 and in the subpopulation CB from 0.019 to 0.490. For this study four SNPs had MAF less than 10% in the subpopulation CS, while, for the subpopulation CB and OPC Just one. In addition, 47.8% of the loci showed MAF ≥ 0.10, 36.9% presented an MAF ≥ 0.20, 23.9% showed an MAF ≥ 0.30 and 8.6% of the evaluated loci presented an MAF ≥ 0.40%. Similar allelic and genotypic frequencies were found in both subpopulations. The average Ho found was 0.358 ± 0.02 and the average value was 0.378 ± 0.02, with greater diversity CB. The majority of the SNPs did not present significant diversions of EHW. Application/Improvements: Finally, the obtained results show that the studied loci have high genetic variability, which is necessary to preserve.
Keywords: Polymorphism, OPC, allelic frequencies, SNPs, genetic diversity.
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