Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn

Eliana Marcela Soto Rueda; Rodrigo Andr eacute s Restrepo; Nelsy Loango; Beatriz Restrepo; Patricia Land aacute zuri    and Mahendra Rai

doi:10.17485/ijst/2019/v12i1/139859

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Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2019/v12i1/139859

Year: 2019, Volume: 12, Issue: 1, Pages: 1-6

Original Article

Potential Anti-Cancer, Cytotoxic and Antioxidant of Polar and Non-Polar Extracts of Origanummajorana Linn

Eliana Marcela Soto Rueda^1,2, Rodrigo Andrés Restrepo¹ , Nelsy Loango^{1, 2}, Beatriz Restrepo¹ , Patricia Landázuri^{1 *} and Mahendra Rai³

¹Facultad de Ciencias de la Salud- Universidad del Quindío, Quindío, Colombia; [email protected], [email protected], [email protected], [email protected], [email protected]
²Programa de Biología- Facultad de Ciencias Básicas- Universidad del Quindío, Quindío, Colombia
³Department of Biotechnology, SGB Amravati University, Amravati - 444602, Maharashtra, India; [email protected]

*Author for correspondence
Patricia Landázuri
Facultad de Ciencias de la Salud- Universidad del Quindío, Quindío, Colombia
Email: [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objective: To evaluate the anticancer and cytotoxic activity of the Origanum majorana and also to test its antioxidant potential. Methods/Statistical Analysis: The in vitro anticancer and cytotoxic activity of the Polar (PF) and Non-Polar Fraction (NPF) obtained from a total Ethanolic Extract (EE) of O. majorana were evaluated using the murine myeloma cell lines Sp2/O-Ag14 (ATCC: CRL-1581) and VERO-ATCC-CCL81. The cell viability was measured with the Colorimetric Quantification System of the Transmittance Index (CQSTI) method; a simple ANOVA of the CQSTI values of each cell line treated with the different extracts was carried out. Findings: The preliminary phytochemical analysis determined the presence of tannins, flavonoids and terpenes in the fractions. EE and PF showed a strong DPPH free radical scavenging capacity, 88,8 and 85.9% uptake, respectively. EE and PF displayed promising anti anticancer activity for both cell lines, so, EE, PF and NPF concentrations that produced 50% cell viability included 5.7 mg/mL, 5.8 mg/mL and 35.8 mg/mL, respectively. Application/Improvements: For the first time it is shown that the PF from O. majorana a low concentrations affected myeloma cells but not VERO cells. These results indicate that this fraction has an anticancer activity. In addition, our results demonstrated that the PF has a high phenolic content, this could be related to its cytotoxic activity.

Keywords: Anticancer, Antioxidant, Cytotoxic, Extract, Flavonoids, O. marjorana, Phenolic Compounds