Predicting Antioxidant, Neuroprotective, and Toxicity Activity of Murraya koenigii Essential Oil by in silico and ex vivo against Rotenone-induced Model

P Devi Guna Sireesha; M Vidyavathi

doi:10.17485/IJST/v16i31.1568

Article

Predicting Antioxidant, Neuroprotective, and Toxicity Activity of Murraya koenigii Essential Oil by in silico and ex vivo against Rotenone-induced Model

VIEWS 382
PDF 122

Indian Journal of Science and Technology

DOI: 10.17485/IJST/v16i31.1568

Year: 2023, Volume: 16, Issue: 31, Pages: 2409-2418

Original Article

Predicting Antioxidant, Neuroprotective, and Toxicity Activity of Murraya koenigii Essential Oil by in silico and ex vivo against Rotenone-induced Model

P Devi Guna Sireesha¹, M Vidyavathi^1*

¹Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Chittoor, 517502, Andhra Pradesh, India

*Corresponding Author
Email: [email protected]

Received Date:23 June 2023, Accepted Date:27 July 2023, Published Date:16 August 2023

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: To study the antioxidant capacity of Murraya koeingii essential oil (MKEO) phytoconstituents Methods: It was determined by using GCMS. The phytoconstituents were then exposed to molecular docking studies that targeted dopamine D2 to look for neuroprotective effects. The highest percentage content of constituents were selected as ligands and dopamine(6VMS) was retrieved from the protein data bank, the docking was performed using Schrodinger software version 2021-4. The pharmacokinetic profile associated with the selected components were predicted utilizing ADMET lab 2.0 and pkCSM which is a graph-based small molecular pk predictor along with toxicity properties of constituents. The neuroprotective activity of MKEO is also predicted by Ex vivo protocol using rotenone as an inducing agent in mice brain slices. Findings: The antioxidant potentials were nearer to the positive control (Ascorbic acid) which proved the antioxidant capacity of MKEO. The antioxidant enzymes of CAT and GSH were also decreased in the mice brain slices which are treated with rotenone and LPO values were decreased. The levels of dopamine, CAT, and GSH were improved in the groups supplemented with MKEO at different concentrations, along with reduced LPO levels. Novelty: These results are reassuring that both In silico and Ex vivo investigations had greater neuroprotective and antioxidant activities for the phytoconstituents examined from MKEO.

Keywords: Murraya koeingii; Neuroprotective; Antioxidant; Toxicity; Parkinson’s Disease

References

Broome ST, Musumeci G, Castorina AA. PACAP and VIP Mitigate Rotenone-Induced Inflammation in BV-2 Microglial Cells. Journal of Molecular Neuroscience. 2022;72(11):2163–2175. Available from: https://doi.org/10.1007/s12031-022-01968-1
Alharthy KM, Althurwi HN, Albaqami FF, Altharawi A, Alzarea SI, Al-Abbasi FA, et al. Barbigerone Potentially Alleviates Rotenone-Activated Parkinson’s Disease in a Rodent Model by Reducing Oxidative Stress and Neuroinflammatory Cytokines. ACS Omega. 2023;8(5):4608–4615. Available from: https://doi.org/10.1021/acsomega.2c05837
Balakrishnan, Vijayraja, Jo, Ganesan, Su-Kim, Choi. Medicinal Profile, Phytochemistry, and Pharmacological Activities of Murraya koenigii and its Primary Bioactive Compounds. Antioxidants. 2020;9(2):101. Available from: https://doi.org/10.3390/antiox9020101
Gougoulias N, Mashev N. Antioxidant activity and polyphenols content of some herbal teas of Lamiaceae family from Greece and Bulgaria. Oxid Commun. 2015;38(1):25–34. Available from: https://www.researchgate.net/publication/274733022
Pires DEV, Blundell TL, Ascher DB. pkCSM: Predicting Small-Molecule Pharmacokinetic and Toxicity Properties Using Graph-Based Signatures. Journal of Medicinal Chemistry. 2015;58(9):4066–4072. Available from: https://doi.org/10.1021/acs.jmedchem.5b00104
Eguchi K, Velicky P, Hollergschwandtner E, Itakura M, Fukazawa Y, Danzl JG, et al. Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions. Frontiers in Cellular Neuroscience. 2020;14:63. Available from: https://doi.org/10.3389/fncel.2020.00063
Devala G, Rao S, Begum P, Penchalaiah H. In-vitro neuroprotective activity of silibinin against MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) induced neurotoxicity model. 2019. Available from: https://doi.org/10.13040/IJPSR.0975-8232.10(5).2301-05
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):90738–90741. Available from: https://doi.org/10.1016/0003-2697(79)90738-3
Aebi H. Catalase in vitro. Methods in Enzymology. 1984;105:121–126. Available from: https://doi.org/10.1016/s0076-6879(84)05016-3
Srivastava SK, Beutler E. Accurate measurement of oxidized glutathione content of human, rabbit, and rat red blood cells and tissues. Anal Biochem. 1968;25(1):90082–90083. Available from: https://doi.org/10.1016/0003-2697(68)90082-1
Manikkoth S, Sequeira M, Joy AE, Rodrigues R. Assessment of brain dopamine levels to evaluate the role of Tylophora indicaethanolic extract on alcohol induced anxiety in Wistar albino rats. Journal of Young Pharmacists. 2016;8(2):91–95. Available from: https://doi.org/10.5530/jyp.2016.2.6
Tan MA, Sharma N, An SSA. Multi-Target Approach of Murraya koenigii Leaves in Treating Neurodegenerative Diseases. Pharmaceuticals. 2022;15(2):188. Available from: https://doi.org/10.3390/ph15020188
Abeysinghe DT, Alwis DDDH, Kumara KAH, Chandrika UG. Nutritive Importance and Therapeutics Uses of Three Different Varieties (Murraya koenigii, Micromelum minutum, and Clausena indica) of Curry Leaves: An Updated Review. Evidence-Based Complementary and Alternative Medicine. 2021;2021:1–23. Available from: https://doi.org/10.1155/2021/5523252
Flores-Soto ME, Corona-Angeles JA, Tejeda-Martinez AR, Flores-Guzman PA, Luna-Mujica I, Chaparro-Huerta V, et al. β-Caryophyllene exerts protective antioxidant effects through the activation of NQO1 in the MPTP model of Parkinson’s disease. Neuroscience Letters. 2021;742:135534. Available from: https://doi.org/10.1016/j.neulet.2020.135534
Sharma S, Gupta J, Prabhakar PK, Gupta P, Solanki P, Rajput A. Phytochemical Repurposing of Natural Molecule: Sabinene for Identification of Novel Therapeutic Benefits Using<i>In Silico</i>and<i>In Vitro</i>Approaches. ASSAY and Drug Development Technologies. 2019;17(8):339–351. Available from: https://doi.org/10.1089/adt.2019.939
Arya A, Chahal R, Rao R, Rahman MH, Kaushik D, Akhtar MF, et al. Acetylcholinesterase Inhibitory Potential of Various Sesquiterpene Analogues for Alzheimer’s Disease Therapy. Biomolecules. 2021;11(3):350. Available from: https://doi.org/10.3390/biom11030350

Copyright

© 2023 Sireesha & Vidyavathi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)