Indian Journal of Science and Technology
Year: 2016, Volume: 9, Issue: 42, Pages: 1-11
Irina Mikhailovna Donnik1 , Maksim Valeryevich Petropavlovsky2*, Anna Sergeevna Krivonogova1 , Irina Alekseevna Shkuratova2 , Marzena Rola-Łuszczak3 and Jacek Kuzґmak3
1Ural State Agrarian University, Yekaterinburg, Sverdlovsk region, Russian Federation; [email protected], [email protected] 2Ural Scientific Research Veterinary Institute, Yekaterinburg, Sverdlovsk region, Russian Federation; [email protected], [email protected] 3Department of Biochemistry, National Veterinary Research Institute, Pulawy, Poland; [email protected]; [email protected]
*Author for correspondence
Maksim Valeryevich Petropavlovsky
Ural Scientific Research Veterinary Institute, Yekaterinburg, Sverdlovsk region, Russian Federation; [email protected]
Objectives: The study is aimed at investigating the genotypic diversity of the causative agent of Bovine Leukemia, circulating in animal populations from different regions of Russia. Methods: Genotyping and sequencing for samples of 444 bp gene DNA element of cattle HVL isolates of Russian regions were conducted and phylogenetic analysis of the isolated strains was performed. Findings: Blood samples, serum and leukoconcentrate of animal blood and DNA samples – env gene region, 444bp long, of cattle leukemia virus were a subject of investigation. Samples from Belgian subgroup (type B) and Australian subgroups (type A) of leukemia virus were singled out in the studied samples. The results of this study provide an opportunity to study features of leukemic epizootic process in the region. For the first time in the middle Urals in Russia the molecular-genetic structure of the cattle leukemia virus was singled out. Phylogenetic analysis of virus derived from DNA sequencing has allowed classifying the studied strains and comparing them with global isolates. Determination of the virus genotype allows for deeper research to identify the pathogenetic variants of leukemic process development in a particular region – in the middle Urals. Study of genotypic diversity of the cattle leukemia virus opens new opportunities and additional approaches to the development of methods for health improvement in leukemia disadvantaged populations. Applications/Improvements: The data obtained will serve as a base for the creation of highly sensitive, specific primers for effective PCR diagnostics.
Keywords: Cattle, Chain Reaction Method, DNA-sequencing, Genotype, Leukemia Virus, Molecular-genetic Structure, Polymerase
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