• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2009, Volume: 2, Issue: 5, Pages: 52-54

Original Article

Sensitive and validated HPLC method for determination of paclitaxel in human serum.


Determination of paclitaxel in human blood sample by HPLC method using carbamazepine as internal standard is described. The absolute recovery of paclitaxel from human serum was >90% and the limit of quantification was 0.05 µg/ml. The intra-day relative standard deviation (RSD) ranged from 1. 02 to 3.73% at 1.0 µg/ml, 1.16 to 5.03% at 10.0 µg/ml and 3.33 to 5.01% at 50.0 µg/ml. The inter-day RSD were 1.03, 2.16 and 1.95% at 1.0, 10.0, 50.0 µg/ml, respectively. The method can be applied to measure the serum concentration of paclitaxel in pharmacokinetic and elution kinetics.

Keywords: Paclitaxel, human blood sample, serum, HPLC


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