Indian Journal of Science and Technology
DOI: 10.17485/ijst/2009/v2i5.16
Year: 2009, Volume: 2, Issue: 5, Pages: 52-54
Original Article
G. Rajender and N. G. B. Narayanan.
Research & Development Division, Relisys Medical Devices Ltd (RMDL), Hyderabad-501 510, Andhra Pradesh.
*Author for the correspondence:
G. Rajender
Research & Development Division,
Relisys Medical Devices Ltd (RMDL), Hyderabad-501 510, Andhra Pradesh.
E-mail: [email protected]
Determination of paclitaxel in human blood sample by HPLC method using carbamazepine as internal standard is described. The absolute recovery of paclitaxel from human serum was >90% and the limit of quantification was 0.05 µg/ml. The intra-day relative standard deviation (RSD) ranged from 1. 02 to 3.73% at 1.0 µg/ml, 1.16 to 5.03% at 10.0 µg/ml and 3.33 to 5.01% at 50.0 µg/ml. The inter-day RSD were 1.03, 2.16 and 1.95% at 1.0, 10.0, 50.0 µg/ml, respectively. The method can be applied to measure the serum concentration of paclitaxel in pharmacokinetic and elution kinetics.
Keywords: Paclitaxel, human blood sample, serum, HPLC
Subscribe now for latest articles and news.
