Indian Journal of Science and Technology
DOI: 10.17485/ijst/2019/v12i13/142597
Year: 2019, Volume: 12, Issue: 13, Pages: 1-5
Original Article
Sajda Khan Gajdhar1*, Shaiq Gajdhar2, Rahul B Patil3 and Smita Chaware4
1Department of Oral Basic and Clinical Sciences, Ibn Sina National College for Medical Studies, Jeddah 22421, Kingdom of Saudi Arabia; [email protected]
2Department of Oral and Maxillofacial Rehabilitation, Ibn Sina National College for Medical Studies, Jeddah 22421, Kingdom of Saudi Arabia; [email protected]
3Department of Oral pathology and Microbiology, Rural dental college, Loni − 414001, Maharashtra, India; [email protected]
4Department of Oral Pathology and Microbiology, Maharashtra Institute of Dental Sciences & Research (MIDSR) Dental College, Latur − 413531, Maharashtra, India; [email protected]
*Author for correspondence
Sajda Khan Gajdhar
Department of Oral Basic and Clinical Sciences, Ibn Sina National College for Medical Studies, Jeddah 22421, Kingdom of Saudi Arabia.
Email: [email protected]
Objectives: To explore the polarizing color profile in connective tissue stroma of Picro-Sirius Red (PSR) stained sections of benign and malignant salivary gland tumors (SGT) and to assess the possible utility of this method as a diagnostic tool. Materials and Methods: A total number of 60 Paraffin blocks of benign (30 cases) and malignant (30 cases) salivary gland tumors were selected from the archives of our institute and regional cancer hospital. 30 cases of Mucous Extravasation Phenomenon (MEP) were selected as control. Salivary gland tumors and control were stained with PSR stain and examined under polarizing microscope. We counted 50 thin and 50 thick collagen fibers in each section of benign and malignant SGT and were categorized as Greenish Yellow (GY) or Yellowish Orange (YO). Statistical analysis was performed by using oneway ANOVA and Paired t-test. Results: We found similar presentation of polarizing color arrangement for thin collagen fibers (80-85% GY and 15-20% YO) in both benign and malignant SGT and MEP. In malignant SGT, thick collagen fibers exhibited different polarizing color (80% GY) than benign SGT and MEP (12-14% GY). Polarizing color profile of collagen in malignant SGT displayed a different presentation as compared to benign SGT and control (MEP) and differences were statistically significant(p<0.05). Conclusion: This study concluded about the difference of stromal collagen fibers in benign and malignant SGT. Malignant SGT suggesting loosely arranged collagen fibers could be involved in further growth and invasion of disease along with other cellular and molecular events.
Keywords: Collagen, Mucous Extravasation Phenomenon, Picro-Sirius Red (PSR) Stain, Polarized Microscopy, Salivary Gland Tumor
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