Indian Journal of Science and Technology
DOI: 10.17485/ijst/2019/v12i16/143985
Year: 2019, Volume: 12, Issue: 16, Pages: 1-6
Original Article
Irianti Kurniasari1, Siwi Indarti2 and Donny Widianto3*
1Department of Biotechnology, Sustainable Agricultural Extension Program, Politeknik Pembangunan Pertanian Malang, Jl. Dr. Cipto 144A, Bedali, Lawang, Malang, 65200, Indonesia; [email protected]
2Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora Bulaksumur, Yogyakarta, 55281, Indonesia; [email protected]
3Research Center for Biotechnology, Universitas Gadjah Mada, Jl. Teknika Utara, Barek, Yogyakarta, 55281, Indonesia; [email protected]
*Author for correspondence
Donny Widianto
Research Center for Biotechnology, Universitas Gadjah Mada, Jl. Teknika Utara, Barek, Yogyakarta, 55281, Indonesia.
Email: [email protected]
Objective: To findout the existence and diversity of bacteria having bio control ability against Globodera rostochiensis from six different location of potato cultivation lands in Wonosobo and Banjarnegara Indonesia. Methods: The bacteria and cyst of G. rostochiensis were isolated and Walkey-Black titration method were used to analyze the C-organic content from each location. The bio control ability of bacterial isolates was tested against cysts and eggs of G. rostochiensis followed by PCR-RAPD with two universal primers for molecular characters differentiation. Simpson’s and Shannon’s index methods were used to measure the diversity of bacteria. Findings: 45 bacterial strains were isolated. The bio control ability test showed that all strains have bio control ability against cysts and eggs of G. rostochiensis. Moreover, the PCR-RAPD showed that 33 isolates were categorized as different species. The high and medium diversity indexes indicated that the soil parasitic bacteria against G. rostochiensis have already been evolved diversely in potato cultivation lands. Novelty: The bacterial isolates that having dominant bio control ability against the golden cyst nematode from each location would be of commercial inoculant bionematicide.
Keywords: Bacteria, Biological Control, Golden Cyst Nematode, G. rostochiensis, Potato
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