The usefulness of comet assay in predicting hematopoietic disorders such as myelodysplastic syndrome and acute myeloid leukemia

Natarajan Gajendran; K  Tanaka  and T  S  Kumaravel  nbsp  nbsp

doi:10.17485/ijst/2008/v1i1/4

Article

The usefulness of comet assay in predicting hematopoietic disorders such as myelodysplastic syndrome and acute myeloid leukemia

VIEWS 914
PDF 315

Abstract
Full-Text HTML
Full-Text PDF
How to Cite

Indian Journal of Science and Technology

DOI: 10.17485/ijst/2008/v1i1/4

Year: 2008, Volume: 1, Issue: 1, Pages: 1-4

Original Article

The usefulness of comet assay in predicting hematopoietic disorders such as myelodysplastic syndrome and acute myeloid leukemia

Natarajan Gajendran¹ , K. Tanaka¹ and T. S. Kumaravel¹

^*Department of Biology, Eritrea Institute of Technology, Mainefhi, Eritrea Email: [email protected]
¹ Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan

^*Author for correspondence
Natarajan Gajendran
Department of Biology, Eritrea Institute of Technology, Mainefhi, Eritrea Email: [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The usefulness of ‘comet assay’ in predicting the hematopoietic disorders such as MDS and AML is reported. Bone marrow biopsies of de novo AML and MDS (3 from each) were subjected to alkaline comet assay along with suitable control. The results are based on the average tailmoment of about hundred randomly selected cells. Blood malignant samples showed significantly higher mean tailmoment (AML: 195±41.6 & MDS: 501±136.2) than the normal (29.66±6.1). In addition, the spontaneous occurrence of apoptotic cells was much higher in MDS (24.5% ±5); while in AML it was <1%. 'Comet assay' offers as single-step visual method to predict the hematopoietic neoplasia in suspected samples.

Keywords: myelodysplastic syndrome, acute myeloid leukemia, comet assay, apoptosis