Indian Journal of Science and Technology
Year: 2008, Volume: 1, Issue: 1, Pages: 1-4
Natarajan Gajendran1 , K. Tanaka1 and T. S. Kumaravel1
*Department of Biology, Eritrea Institute of Technology, Mainefhi, Eritrea Email: [email protected]
1 Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan
*Author for correspondence
Department of Biology, Eritrea Institute of Technology, Mainefhi, Eritrea Email: [email protected]
The usefulness of ‘comet assay’ in predicting the hematopoietic disorders such as MDS and AML is reported. Bone marrow biopsies of de novo AML and MDS (3 from each) were subjected to alkaline comet assay along with suitable control. The results are based on the average tailmoment of about hundred randomly selected cells. Blood malignant samples showed significantly higher mean tailmoment (AML: 195±41.6 & MDS: 501±136.2) than the normal (29.66±6.1). In addition, the spontaneous occurrence of apoptotic cells was much higher in MDS (24.5% ±5); while in AML it was <1%. 'Comet assay' offers as single-step visual method to predict the hematopoietic neoplasia in suspected samples.
Keywords: myelodysplastic syndrome, acute myeloid leukemia, comet assay, apoptosis
Subscribe now for latest articles and news.