Indian Journal of Science and Technology
Year: -0001, Volume: 11, Issue: 24, Pages: 1-14
Darwin Hernandez1 *, Donicer Montes1 and Luz Angela Alvarez2
Objectives: To associate the BoLA-DRB3 alleles with the resistance and/or susceptibility to the development of the Enzootic Bovine Leukosis (EBL), in the Harton del Valle cattle. Methods: In 93 animals of the Harton del Valle breed, the infection with the Bovine Leukosis Virus (BLV), the development of Persistent Lymphocytosis (PL), the production of antibodies against the virus (anti-BLV) for one year were evaluated. The animals were genotyped for the BoLA-DRB3 locus. The number of alleles and their frequencies was estimated. Alleles were associated with infection with BLV (env+/-), development of PL (PL+/-) and production of anti-VL (anti-BLVhigh/low) using Odds Ratio (OR), the alleles were classified as Resistant (R), Neutral (N) or Susceptible (S) to the variables evaluated. Additionally, the sequences were explored in order to find common amino acid motifs. Findings: We found 27 alleles BoLA-DRB3, the most frequent alleles were *1101 (14.5%), *0902 (7%) and *2703 (7%), *20012 (6.5%), *1501 (5.9%) and *1701 (5.9%) The remaining 21 alleles had a cumulative frequency of 53.2%. The alleles *1001, *1101, *2006, *2703, *3001 and *4802 were considered as Renv infection with the virus, whereas, the alleles *0902, *1601, *1701, *20012 and *25011 were they considered Senv. Only the allele *1101 was significantly associated with the absence of PL and the alleles *25011 and *2703 were considered SPL. The alleles *1101 and *2703 were Ranti-BLV, while, the alleles *1601 and *1701 were classified as Santi-BLV. The allelic frequencies of the R alleles were higher than the S. Some common amino acid motifs were found between alleles with the same classification. Only the allele *1101 was Renv/PL/anti-BLV. The protective effect of the allele was greater in the heterozygous state (OR = 0.18, p<0.00005). Application/Improvements: The BoLA-DRB3 locus is highly polymorphic. The allele *1101 was considered as Renv/PL/anti-BLV. The heterozygote advantage was shown when responding against the development of the disease. The data presented here can be used in future genetic improvement programs aimed at increasing the frequency of alleles of resistance to the disease.
Keywords: Bovine Leucocyte Antigens, Colombian Creole Cattle, Genetic Diversity
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