Indian Journal of Science and Technology

Abstract

Objectives: The current study focuses on characterization and isolation of bioactive compounds from a marine halophilic bacterium and testing its antimicrobial potentiality against clinically important pathogens like Proteus mirabilis, Salmonella typhi A, Salmonella typhi B, Pseudomonas aeruginosa and Klebsiella pneumoniae. The study also portraits presence of possible bioactive compounds responsible for such antimicrobial activity through Gas Chromatography-Mass Spectrometry (GC-MS). Methodology: Surface water samples were collected from Kovalam beach at Thiruvananthapuram, Kerala state, a South Indian coast of Indian peninsula and were screened for potential bacteria. Morphological, biochemical and molecular profiling were performed to identify and isolate the bacterium of interest. Antibiogram studies were conducted to understand pathogenic trends in the isolate. Culture supernatant of the test bacterium was screened against clinically important pathogens. Further, crude extract of the culture supernatant was subject to GC-MS analysis. Findings: Morphological, biochemical and molecular profiling of the isolate revealed the organism to be Bacillus subtilis. The culture supernatant had significant activity against clinically important pathogens. GC-MS analysis revealed possible antimicrobial compound to be (Pyrrolo [1, 2-a] pyrazine-1, 4-dione). Novelty: The study has significant importance as it depicts antimicrobial activity of halophiles against potent clinical pathogens.

Keywords: Antibiogram Profiling, Bioactive Compounds, Gas Chromatography-Mass Spectrometry (GC-MS), Marine Halophiles, Molecular Profiling