Indian Journal of Science and Technology
Year: 2016, Volume: 9, Issue: 9, Pages: 1-5
R. Indira Iyer1* , C. Selvaraju2 and S. T. Santhiya1
1Department of Genetics, Dr. ALMPGIBMS, University of Madras (Taramani Campus), Chennai - 600113, Tamil Nadu, India; [email protected], [email protected] 2National Centre for Ultrafast Processes, Dr. ALMPGIBMS, University of Madras (Taramani Campus), Chennai- 600113, Tamil Nadu, India; [email protected]
*Author for Correspondence
R. Indira Iyer Department of Genetics, Dr. ALMPGIBMS, University of Madras (Taramani Campus), Chennai - 600113, Tamil Nadu, India; [email protected]
Objectives: To study the potential of callus cultures of Vigna radiata for the biosynthesis of silver nanoparticles. Methods: Callus cultures of Vigna radiata were established in MS media and the aqueous extract of callus cultures was used for biogenic synthesis of silver nanoparticles using 1 mM AgNO3 at ambient temperature.The nanoparticles were characterized by UV-visible spectroscopy and scanning electron microscopy. Findings: This is the first report of use of callus cultures of Vigna radiata for the biogenesis of silver nanoparticles.The synthesis was rapid and completed in 2 hours. UV-visible spectrophotometry revealed an absorbance peak at 455 nm. Scanning electron microscopy revealed the presence of a high density of spherical or ellipsoidal silver nanoparticles. Phytochemical studies revealed the presence of phenolics in the extract which could have played a role in the formation of silver nanoparticles.The findings are important since this is a novel approach and there are very few reports of synthesis of silver nanoparticles using callus cultures. Application: Callus cultures are more suitable for biosynthesis of bio-compatible nanoparticles as compared to microbes or plant parts since these are generated axenically and are renewable resources that are easy to authenticate.
Keywords: Callus, Green Gram, Silver Nanoparticles, Vigna radiata
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