Cluster Analysis of Clinical, Food, and Handler Methicillin-Resistant Staphylococcus Aureus Isolates Characterized by Pulse Field Gel Electrophoresis

Tagreed Sabeeh Abdulla; Saad Sabah Fakhry; Sundus Ali Jassin; Farqad Abdullah Rashid; Ali Abdel Qader Abdulbaqi

doi:10.17485/IJST/v15i41.15

Article

Cluster Analysis of Clinical, Food, and Handler Methicillin-Resistant Staphylococcus Aureus Isolates Characterized by Pulse Field Gel Electrophoresis

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Indian Journal of Science and Technology

DOI: 10.17485/IJST/v15i41.15

Year: 2022, Volume: 15, Issue: 41, Pages: 2171-2181

Original Article

Cluster Analysis of Clinical, Food, and Handler Methicillin-Resistant Staphylococcus Aureus Isolates Characterized by Pulse Field Gel Electrophoresis

Tagreed Sabeeh Abdulla¹, Saad Sabah Fakhry^1*, Sundus Ali Jassin¹, Farqad Abdullah Rashid¹, Ali Abdel Qader Abdulbaqi¹

¹Food Contamination Research Center, Food Contamination Research Center at the Ministry of Science and Technology, Directorate of Environment and Water, Al Jadyria, Baghdad, Iraq

*Corresponding Author
Email: [email protected]

Received Date:04 January 2022, Accepted Date:25 May 2022, Published Date:08 November 2022

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: To identify and to characterize of methicillin-resistant Staphylococcus aureus (MRSA) isolates collected from food (n=43), food handler (n=8), and clinical samples (n=15). Methods: Genotyping was applied for the purposes of epidemiological investigation and source tracking of these isolates. Pulsed field gel electrophoresis (PFGE) was performed using the restriction endonucleases SmaI. Findings: PFGE analysis showed that pulse types of isolates from clinical samples differed that from food and food handler isolates. However, a similarity was observed between pulse types of isolates from food and food handlers, suggesting an epidemiological association between isolates from these two sources and that MRSA may be transmitted from food handlers to foods. Novelty : PFGE genotyping of MRSA isolates was useful, as a first study of its type in Iraq, for investigating epidemiological relatedness and even contaminated food sources.

Keywords: Epidemiology; MRSA isolates; PFGE; SmaI

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© 2022 Abdulla et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)