Indian Journal of Science and Technology
Year: 2009, Volume: 2, Issue: 12, Pages: 50-54
G. Baskar and S.Renganathan1
Department of Biotechnology, St. Joseph’s College of Engineering, Chennai–600 119, India.
1Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai - 600 025, India.
*Author for the correspondence:
Department of Chemical Engineering, Alagappa College of Technology, Anna University, Chennai - 600 025, India.
E-mail: [email protected]
The effect of ammonium chloride, urea and sodium nitrate as supplementary nitrogen source on the production of extracellular L-asparaginase using Aspergillus terreus MTCC 1782 was investigated using 5- level Latin square design. The statistical reliability and significance of the variables was studied by performing ANOVA for experimental L-asparaginase activity using Dataplot software. Among the supplementary nitrogen sources studied, ammonium chloride was found to be the best for maximum L-asparaginase activity (confidence level of 96.61%) with less biomass formation (confidence level of 21.93%). The maximum L-asparaginase activity obtained was of 26.47 IU/mL by A. terreus MTCC 1782 using groundnut oil cake powder as natural substrate in submerged fermentation.
Keywords: Aspergillus terreus, L-asparaginase, latin square design
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