Indian Journal of Science and Technology
DOI: 10.17485/ijst/2015/v8i9/65704
Year: 2015, Volume: 8, Issue: 9, Pages: 804–813
Original Article
B. Pramila1,2, P. Kalaivani1 , R. Barathidasan1 and C. Saravana Babu1*
1 Centre for Toxicology and Developmental Research, Sri Ramachandra University, Porur, Chennai - 600 116, Tamil Nadu, India; pramilaceft@gmail.com, kalaiperiyathambi@gmail.com, barathdassss@gmail.com, ceftpublications@gmail.com
2 Department of Biotechnology, Dr. M.G.R. Educational and Research Institute University, Maduravoyal, Chennai - 600 095, Tamil Nadu, India
Objectives: To investigate the neuroprotective effect of combination of Citicoline (membrane stabilizer) and L-NAME (NOS inhibitor) in middle cerebral artery occluded/reperfused rats (MCAO/R). Methods: Citicoline (250 mg/kg b.w.) followed by L-NAME (3 mg/kg b.w.) was administered 30 minutes before (Pre) performing middle cerebral artery occlusion/reperfusion (MCAO/R) in Sprague Dawley rats. Parameters such as Glutamate, Aspartate, Nitrite/Nitrate, Sodium potassium ATPase (Na+ K+ ATPase) content, expression of TNF- α and IL-10, and histopathology was studied. Neurological scoring was also performed in the MCAO/R rats. Findings: Pre ischemic administration of citicoline and L-NAME has improved neurological deficit (p<0.001). Combination treatment has also ameliorated the glutamate level in MCAO/R rats (p<0.05). Combination of these drugs has also reduced the nitrate/nitrite content (p<0.001) and also sodium potassium ATPase (Na+ K+ ATPase) (p<0.001) significantly. Citicoline and L-NAME combination treatment has also down regulated the expression of TNF-α and up regulated the expression of IL-10 in middle cerebral artery occlusion/reperfusion rats. Citicoline and L-NAME combination was also observed to reduce the neuronal damage which is evident from the histopathology (H and E) of brain sections of MCAO/R rats. Novelty of the Study: Though Citicoline and L-NAME has been evaluated and proven for their neuroprotection separately, the effect of these two drugs when administered together as a combination has not been studied. This study is first of its kind to determine the neuroprotective effect of combination of citicoline and L-NAME. Conclusion/Application: Combination of citicoline and L-NAME has profoundly improved neuroprotection and ameliorated excitotoxicity when administered 30 minutes before ischemia.
Keywords: Cerebral Ischemia, Citicoline, L-NAME, Middle Cerebral Artery Occlusion/Reperfusion, Transient Focal Cerebral Ischemia
Subscribe now for latest articles and news.