Cytotoxicity Testing of Dental Materials

K R Navaneethakrishnan; Sanjey Kumar Prabath Udaya Kumar; M R Murali; T N Sathya; Sangeetha V Naveen; S S Murugan; T S Kumaravel

doi:10.17485/IJST/v16i27.KRN

Article

Cytotoxicity Testing of Dental Materials

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Indian Journal of Science and Technology

DOI: 10.17485/IJST/v16i27.KRN

Year: 2023, Volume: 16, Issue: 27, Pages: 2035-2039

Original Article

Cytotoxicity Testing of Dental Materials

K R Navaneethakrishnan¹, Sanjey Kumar Prabath Udaya Kumar^2,3, M R Murali¹, T N Sathya¹, Sangeetha V Naveen¹, S S Murugan⁴, T S Kumaravel^5*

¹Toxicologist, Department of Toxicology, GLR Laboratories Private Limited, India
²Summer Intern, GLR Laboratories Private Limited, India
³Student, College of Life Sciences and Biotechnology, Korea University, Korea
⁴Toxicologist and Managing Director, GLR Laboratories Private Limited, India
⁵Toxicologist and Chairman, GLR Laboratories Private Limited, India

*Corresponding Author
Email: [email protected]

Received Date:25 November 2022, Accepted Date:19 June 2023, Published Date:11 July 2023

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives: In this manuscript, we aim to review and evaluate the cytotoxicity data of 16 dental materials that have been generated in our laboratory over the past five years. Methods: Cytotoxicity data of dental materials generated in our laboratory over the past five years were retrieved from the archives. We collected and analyzed detailed information regarding the dental materials, tests conducted, regulatory standards followed, and the results obtained. Findings: Our laboratory conducted tests on a total of 16 dental materials over the course of the past five years, which included both metals and polymers. The commonly employed regulatory standard for these tests was ISO 10993-5 and ISO 7405. We utilized various methodologies such as elution, direct contact, agar diffusion, and colony formation assays. Out of the 16 materials tested, 15 were found to be non-cytotoxic. However, during the agar diffusion assay, one specific material, namely Oxyalkylene polymer with synthetic diamond powder, exhibited moderate cytotoxicity. Novelty: This study contributes to the existing body of knowledge by providing detailed information on the cytotoxicity profiles of certain dental materials, including both metals and polymers.

Keywords: Dental Biomaterials; ISO 7405; ISO 109935; Biocompatibility; Cytotoxicity

References

Kassapidou M, Hjalmarsson L, Johansson CB, Johansson PH, Morisbak E, Wennerberg A, et al. Cobalt–chromium alloys fabricated with four different techniques: Ion release, toxicity of released elements and surface roughness. Dental Materials. 2020;36(11):e352–e363. Available from: https://doi.org/10.1016/j.dental.2020.08.012
Clark D, Levin L. Prognosis and complications of mature teeth after lateral luxation. The Journal of the American Dental Association. 2019;150(8):649–655. Available from: https://doi.org/10.1016/j.adaj.2019.03.001
Matusiewicz H, Richter M. Metal ions release from metallic orthopedic implants exposed to tribocorrosion and electrochemical corrosion conditions in simulated body fluids: Clinical context and in vitro experimental investigations. World Journal of Advanced Research and Reviews. 14(2):261–283. Available from: https://doi.org/10.30574/wjarr.2022.14.2.0438
Sarraf M, Ghomi ER, Alipour S, Ramakrishna S, Sukiman NL. A state-of-the-art review of the fabrication and characteristics of titanium and its alloys for biomedical applications. Bio-Design and Manufacturing. 2022;5(2):371–395. Available from: https://doi.org/10.1007/s42242-021-00170-3
Saulacic N, Schaller B. Prevalence of Peri-Implantitis in Implants with Turned and Rough Surfaces: a Systematic Review. PMCID. 2019. Available from: https://doi.org/10.5037/jomr.2019.10101
Hanawa T. Biocompatibility of titanium from the viewpoint of its surface. Science and Technology of Advanced Materials. 2022;23(1):457–472. Available from: https://doi.org/10.1080/14686996.2022.2106156
Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity. ISO. 2009. Available from: https://www.iso.org/standard/36406.html
Dentistry - Evaluation of biocompatibility of medical devices used in dentistry ISO 7405. 2018. Available from: https://www.iso.org/obp/ui#iso:std:iso:7405:dis:ed-3:v1:en
Weinstein T, Clauser T, Fabbro D, Deflorian M, Parenti M, Taschieri A, et al. Prevalence of Peri-Implantitis: A Multi-Centered Cross-Sectional Study on 248 Patients. Dent J (Basel). 2020;8(3):7557736. Available from: https://doi.org/10.3390/dj8030080
Warren EK, Vaddi A, Tadinada A. A comparative evaluation of the metallic artifact generated by a ceramic dental implant and a titanium dental implant imaged on cone-beam computed tomographic scans. JADA Foundational Science. 2022;1:100007. Available from: https://doi.org/10.1016/j.jfscie.2022.100007
Grosgogeat B, Vaicelyte A, Gauthier R, Janssen C, Borgne ML. Toxicological Risks of the Cobalt–Chromium Alloys in Dentistry: A Systematic Review. Materials. 15(17):5801. Available from: https://doi.org/10.3390/ma15175801
Stricker A, Bergfeldt T, Fretwurst T, Addison O, Schmelzeisen R, Rothweiler R, et al. Impurities in commercial titanium dental implants - A mass and optical emission spectrometry elemental analysis. 2022. Available from: https://doi.org/10.1016/j.dental.2022.06.028
Biological evaluation of medical devices - Part 1: Evaluation and testing within a risk management process. 2018. Available from: https://www.iso.org/standard/68936.html
Kostić M, Nikolić L. Contemporary Dental materials. 2019. Available from: https://scindeks-clanci.ceon.rs/data/pdf/2406-2979/2019/2406-29791901078K.pdf
Velasco-Ibáñez R, Lara-Carrillo E, Morales-Luckie RA, Romero-Guzmán ET, Toral-Rizo VH, Ramírez-Cardona M, et al. Evaluation of the release of nickel and titanium under orthodontic treatment. Scientific Reports. 2020;10(1):22280. Available from: https://doi.org/10.1038/s41598-020-79221-1

Copyright

© 2023 Navaneethakrishnan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)