• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2023, Volume: 16, Issue: 27, Pages: 2035-2039

Original Article

Cytotoxicity Testing of Dental Materials

Received Date:25 November 2022, Accepted Date:19 June 2023, Published Date:11 July 2023


Objectives: In this manuscript, we aim to review and evaluate the cytotoxicity data of 16 dental materials that have been generated in our laboratory over the past five years. Methods: Cytotoxicity data of dental materials generated in our laboratory over the past five years were retrieved from the archives. We collected and analyzed detailed information regarding the dental materials, tests conducted, regulatory standards followed, and the results obtained. Findings: Our laboratory conducted tests on a total of 16 dental materials over the course of the past five years, which included both metals and polymers. The commonly employed regulatory standard for these tests was ISO 10993-5 and ISO 7405. We utilized various methodologies such as elution, direct contact, agar diffusion, and colony formation assays. Out of the 16 materials tested, 15 were found to be non-cytotoxic. However, during the agar diffusion assay, one specific material, namely Oxyalkylene polymer with synthetic diamond powder, exhibited moderate cytotoxicity. Novelty: This study contributes to the existing body of knowledge by providing detailed information on the cytotoxicity profiles of certain dental materials, including both metals and polymers.

Keywords: Dental Biomaterials; ISO 7405; ISO 109935; Biocompatibility; Cytotoxicity


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© 2023 Navaneethakrishnan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)


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