Indian Journal of Science and Technology
Year: 2022, Volume: 15, Issue: 42, Pages: 2240-2251
Shalini Sehgal1, Sunita Aggarwal2*, Nitika Nagpal2, Aparajita Sen3, Aafreen2, Aishani Chakraborty2, Mrinalini Venugopal2, Rhea Bhalla2, Yashaswi Rawat2
1Department of Food Technology, Bhaskaracharya College of Applied Sciences, University of Delhi, New Delhi, India
2Department of Microbiology, Institute of Home Economics, University of Delhi, New Delhi, India
3Department of Genetics, University of Delhi South Campus, New Delhi, India
Email: [email protected]
Received Date:13 June 2021, Accepted Date:25 September 2022, Published Date:14 November 2022
Objective: To review the current diagnostic methodology available for the Coronavirus Disease 2019 (COVID-19) caused by new Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Method: This review critically analyses the merits and limitations of the recent COVID-19 diagnostics being used for disease prophylaxis and its mitigation. Multiple research papers from 2019- 2022 were consulted. Many novel diagnostics have been included, such as LAMP, CRISPR, AI and other POC techniques, along with conventional RTPCR and CT -SCAN. These have been compared based on principle, protocol, sensitivity, specificity, cost-effectiveness and their pros and cons. Findings: Mass Spectrometry and Loop mediated isothermal amplification (LAMP) were seen to be the most sensitive and specific. They were also very rapid. However, Mass Spectrometry is expensive, as it requires sophisticated instrumentation. LAMP on the other hand, does not require expensive machinery, and thus is a better choice. Novelty: This review has covered most of the techniques, which were not earlier covered in reviews. We have compiled all data in one manuscript for the ease of readers. We have also talked about the diagnostic protocols made in India and compared their sensitivities and specificities.
Keywords: COVID-19; Diagnostics; Coronavirus; Pandemic; Vaccine; Therapeutic
© 2022 Sehgal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)
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