Indian Journal of Science and Technology
DOI: 10.17485/IJST/v16i11.1458
Year: 2023, Volume: 16, Issue: 11, Pages: 775-784
Original Article
Sujata Yadav1*, Geetika Wag1, Ashwini Kumar Gupta2
1Research Scholar, School of Studies in Life Science, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
2Professor, School of Studies in Life Science, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, India
*Corresponding Author
Email: [email protected]
Received Date:30 August 2022, Accepted Date:07 October 2022, Published Date:15 March 2023
Objectives: To explore the endophytic actinobacteria from the root tissue of Bryophyllum pinnatum (Lam.) Oken and its assessment for antimicrobial and antibiofilm activity against human pathogens. Methods: Surface sterilized root tissues of B. pinnatum have been seeded in different isolation media, to obtain colonies of endophytic actinobacteria. A polyphasic approach has been used to identify the isolate. Cross streak and disc diffusion methods have been used to perform the antimicrobial activity. However, the crystal violet assay assessed the antibiofilm activity, and its visualization was done under the light and fluorescent microscope. Findings: By polyphasic approach, Actinobacteria strain BpR-2 GER has been confirmed to be the member of genus Streptomyces, further through 16S rRNA gene sequence analysis, the strain has been identified as Streptomyces rochei that has 100% sequence similarity with Streptomyces rochei NRRL B-2410 (MUMD01000370). The isolate has exhibited extensive antagonistic interaction with Clinical and MTCC (Microbial Type Culture Collection) pathogens ranging from 9.750.71 to 19.381.30 mm. The highest zone of inhibition was recorded against Gram-positive bacteria B. subtilis (MTCC-441) of 19.381.30 mm and Gram-negative bacteria Proteus mirabilis (Clinical) of 16.250.71 mm. The strain has also exhibited significant antibiofilm potential against the MTCC-7443 (85.130.003 to 88.280.003%) and Clinical (85.130.003 to 88.280.003%) strain of Staphylococcus aureus. Novelty: This is the first report of S. rochei BpR-2 GER, a root endophyte of B. pinnatum with broad-spectrum antimicrobial activity and a significant antibiofilm property.
Keywords: Antimicrobial activity; Antibiofilm activity; Bryophyllum pinnatum; Endophytic Actinobacteria; Streptomyces rochei
© 2023 Yadav et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Published By Indian Society for Education and Environment (iSee)
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