Indian Journal of Science and Technology

Abstract

Background/Objectives: To understand the genotoxicity and cytotoxicity potentials in tobacco-chewing habitues from coastal Andhra Population. Place and Duration of Study: Translational Research Institute of Molecular Sciences, between October 2013 to October 2014.
Methods/Statistical Analysis: Present study was carried out using 35 individuals (28 men, 7 women; age range 25-56 years) who are having a habit of chewing tobacco from 5-20 years were tested along with 35 controls with same age group. Samples were cultured and stained with Hoechst 3325. Scored chromosomal abnormalities, SCE’s, CPK and MI using statistical evaluations. Results/Findings: In this study we have scored the numerical and structural abnormalities, Mitotic Index (MI), Cell Proliferation Kinetics (CPK) and Sister Chromatid Exchanges (SCE’s) of the presented samples have been calculated. Although significant numerical aberration observed, structural abnormalities were notably seen in a few cases. When compared with controls tobacco-chewing habitués does increase induced significant mito depression. Our data reveals that samples CPK values were remarkably decreased when compared to control value.
Conclusion/Application: The demonstration of chromosomal damage in lymphocytes from tobacco chewing habitues strongly indicates that tobacco should be categorized as a human carcinogen and major risk factor for oral, throat and pharynx cancers. 

Keywords: BrdU-labelling, CA, CPK, MI, SCE