Fasciola gigantica : Eggshell solubility and permeability properties

P  Balasubramanian; P  Ramasamy     and G  P  Brennan

doi:10.17485/ijst/2010/v3i1.13

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Fasciola gigantica : Eggshell solubility and permeability properties

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Indian Journal of Science and Technology

DOI: 10.17485/ijst/2010/v3i1.13

Year: 2010, Volume: 3, Issue: 1, Pages: 17-20

Original Article

Fasciola gigantica : Eggshell solubility and permeability properties

P. Balasubramanian^1*, P. Ramasamy ^1** and G. P. Brennan ²

¹ Department of Biotechnology, Life Sciences Building, University of Madras, Guindy Campus, Chennai-600025, India.
² School of Biol. & Bioche., Medical Biology Centre, Queens’ Univ., Belfast BT9 7BL, N- Ireland, U.K.
^*Present Address: Department of Biotechnology, VELS University, Pallavaram, Chennai-600117, Tamil Nadu, India
^** Present Address: Vice-chancellor, Alagappa Nagar, Karaikudi-630003, Tamil Nadu, India
[email protected] ; [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The cross-linkages contained within the proteinaceous opercular cement seal holding the egg operculum of Fasciola gigantica solubilised in 0.1N saturated NaOH, concentrated HCl, H_2SO₄ and H₃PO₄, whereas the quinone tanned eggshell solubilised only in concentrated HNO₃ and NaOCl. In TCA, H₃BO₃ and CH₃COOH, the cross linkages of proteinaceous opercular cement and the quinone tanned eggshell remained unaffected. This study has shown that the cross linkages of the opercular cement, the operculum and the quinone-tanned eggshell differ in their permeability and solubility properties.
Keywords: Fasciola gigantica, eggshell, permeability and solubility in acids, alkali and chemicals