• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2020, Volume: 13, Issue: 19, Pages: 1927-1934

Original Article

Screening of biosurfactant producing yeasts isolated from mangrove ecosystem of Surat region of Gujarat, India

Received Date:08 April 2020, Accepted Date:23 April 2020, Published Date:18 June 2020


Background/ Objectives: Biosurfactants are extracellular surface-active compounds produced by bacteria, fungi and yeast. Interest in biosurfactant has been increasing due to their unique properties. The objective of this study is to screen for potential biosurfactant producing yeasts from mangrove areas of Gujarat in India. Methodology: The biosurfactant producing yeasts isolated from five different mangrove sites i.e. Hazira, Mandroi, Mirzapur, Kantiyajal and Machhad of Surat, Gujarat during summer season of 2015. Isolated yeasts were screened for the production of biosurfactant by Parafilm M, Oil displacement and Emulsification index method in which cottonseed oil was used as a substrate. The type of biosurfactant produced was identified by Phenol-sulfuric acid test, Biuret test and phosphate test. Findings: Total twenty four yeasts were isolated from soils of sampling sites. Yeasts were initially screened by Parafilm M and oil displacement method. Out of these 24 isolate, 6 isolates namely, Ky-48, Ky-53, Ky-54, Ky-84, Ky-86 and Ky-87 showed promising biosurfactant activity. These six isolates were further subjected to secondary screening method: Emulsification test, to identify biosurfactant production. Two isolates, namely Ky-46 (emulsification index: 61.53 %) and Ky-86 (emulsification index: 46.66 %) showed maximum biosurfactant production. All six isolates showed positive result for phenol- sulfuric acid method. This indicated that the isolated biosurfactant was Glycolipid in nature. Novelty/Applications: Few mangrove yeasts have potential to produce high amount of glycolipid that can be used in food processing and detergents, healthcare and cosmetics industries.

Keywords: Biosurfactant; emulsification index; oil displacement; glycolipid


  1. Saharan B, Sahu R, Sharma D. A Review on Biosurfactants: Fermentation, Current Developments and Perspectives. Genetic Engineering and Biotechnology Journal. 2011;2011(1).
  2. Banat I, Thavasi R, Jayalakshmi S. Biosurfactants from marine bacterial isolates. InCurrent research, technology and education topics in applied microbiology and microbial biotechnology. Available from: https://pure.ulster.ac.uk/en/publications/biosurfactants-from-marine-bacterial-isolates-3
  3. Zajic JE, Guignard H, Gerson DF. Properties and biodegradation of a bioemulsifier from Corynebacterium hydrocarboclastus. Biotechnology and Bioengineering. 1977;19(9):1303–1320. doi: 10.1002/bit.260190905
  4. Ilori MO, Amund DI. Production of a Peptidoglycolipid Bioemulsifier by Pseudomonas aeruginosa Grown on Hydrocarbon. Zeitschrift für Naturforschung C. 2001;56(7-8):547–552. doi: 10.1515/znc-2001-7-812
  5. Luna JMd, Sarubbo L, Campos-Takaki GMd. A new biosurfactant produced by Candida glabrata UCP 1002: characteristics of stability and application in oil recovery. Brazilian Archives of Biology and Technology. 2009;52(4):785–793. doi: 10.1590/s1516-89132009000400001
  6. Desai JD, Banat IM. Microbial production of surfactants and their commercial potential. Microbiology and molecular biology reviews : MMBR. 1997;61(1):47–64. doi: 10.1128/.61.1.47-64.1997
  7. Kosaric N. Biosurfactants in industry. Pure and Applied Chemistry. 1992;64(11):1731–1737. doi: 10.1351/pac199264111731
  8. Jain RM, Mody K, Joshi N, Mishra A, Jha B. Production and structural characterization of biosurfactant produced by an alkaliphilic bacterium, Klebsiella sp.: Evaluation of different carbon sources. Colloids and Surfaces B: Biointerfaces. 2013;108:199–204. doi: 10.1016/j.colsurfb.2013.03.002
  9. Silva ACSd, Santos PNd, Silva TALe, Andrade RFS, Campos-Takaki GM. Biosurfactant production by fungi as a sustainable alternative. Arquivos do Instituto Biológico. 2018;85(0). doi: 10.1590/1808-1657000502017
  10. Cameron DR, Cooper DG, Neufeld RJ. The mannoprotein of Saccharomyces cerevisiae is an effective bioemulsifier. Applied and Environmental Microbiology. 1988;54(6):1420–1425. doi: 10.1128/aem.54.6.1420-1425.1988
  11. Cooper DG, Paddock DA. Production of a Biosurfactant from Torulopsis bombicola. Applied and Environmental Microbiology. 1984;47(1):173–176. doi: 10.1128/aem.47.1.173-176.1984
  12. Fontes GC, Amaral PFF, Coelho MAZ. Produção de biossurfactante por levedura. Química Nova. 2008;31(8):2091–2099. doi: 10.1590/s0100-40422008000800033
  13. Kitamoto D, Haneishi K, Nakahara T, Tabuchi T. Production of mannosylerythritol lipids by Candida antarcticafrom vegetable oils. Agricultural and biological chemistry. 1990;54(1):37–40. Available from: https://doi.org/10.1271/bbb1961.54.37
  14. Kalyani AL, Naga SG, Aditya AK, SG. Isolation and antimicrobial activity of rhamnolipid (biosurfactant) from oil-contaminated soil sample using humic-acid salts-vitamin agar. International Journal of Research in Engineering and Technology. 2014;3(5):357–365.
  15. Morikawa M, Daido H, Takao T, Murata S, Shimonishi Y, Imanaka T. A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS38. Journal of Bacteriology. 1993;175(20):6459–6466. doi: 10.1128/jb.175.20.6459-6466.1993
  16. Satpute SK, Banpurkar AG, Dhakephalkar PK, Banat IM, Chopade BA. Methods for investigating biosurfactants and bioemulsifiers: a review. Critical Reviews in Biotechnology. 2010;30(2):127–144. doi: 10.3109/07388550903427280
  17. Kalyani A, Sireesha GN. Isolation of bio-surfactant producing actinomycetes from terrestrial and marine soils. International journals of Pharmaceuticals Sciences and Research. 2014;5(9):4015–4022. Available from: http://dx.doi.org/10/13040/IJPSR.0975-8232(9)
  18. Mukherjee S, Das P, Sen R. Towards commercial production of microbial surfactants. Trends in Biotechnology. 2006;24(11):509–515. doi: 10.1016/j.tibtech.2006.09.005
  19. Vijaya B, Jayalakshmi NR, Manjunath K. Enumeration of biosurfactant producing microorganisms from oil contaminated soil in and around Bangalore (India) International Journal of Current Science. 2013;5:86–94.
  20. Ianieva O. 2013. Available from: http://nbuv.gov.ua/UJRN/MicroBioll_2013_75_4_10
  21. Chandran PR, Das N. Biosurfactant production and diesel oil degradation by yeast species Trichosporonasahii isolated from petroleum hydrocarbon contaminated soil. International Journal of Engineering Science and Technology. 2010;2(12):6942–53.


© 2020 Patel, Patel. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Published By Indian Society for Education and Environment (iSee)


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