• P-ISSN 0974-6846 E-ISSN 0974-5645

Indian Journal of Science and Technology


Indian Journal of Science and Technology

Year: 2022, Volume: 15, Issue: 22, Pages: 1075-1083

Original Article

Green Synthesis and Characterization of Copper Nanoparticles Using Urginea wightii and its Biological Activities

Received Date:04 February 2022, Accepted Date:04 May 2022, Published Date:22 June 2022


Objectives: To investigate the potential of copper and copper oxide nanoparticles synthesized from the bulb extract of Urginea wightii (U. wightii). Methods: Copper and copper oxide nanoparticles (Cu-Cu2O NPs) were synthesized by the reduction method and were characterized by using UV-vis spectroscopy, FTIR, XRD, SEM, and EDX. Antioxidant activity was calculated by DPPH and ABTS method. In vitro antimicrobial activity by agar well diffusion method and cytotoxic activities by MTT assay. Findings: The presence of Cu-Cu2O NPs was confirmed by visible colour change, XRD pattern showed distinctive peaks corresponding to 110, 111, 200, 220, and 222 planes revealing the crystalline nature; FTIR spectra showed the presence of different functional groups such as alcohol (OH-stretch), alkene (C=C stretching) and amines (NH bending), etc. SEM studies revealed the morphological and structural characteristics of biosynthesized NPs and the percentage of inhibition was 92% and 90% at 100 mg/mL respectively. Further studies of in vitro antimicrobial activity of different concentrations of Cu-Cu2O NPs of U. wightii, showed the highest inhibition zone against Pseudomonas aeruginosa (22 mm). The NPs exhibited cytotoxic activity against the A549 lung cancer cell line in a dose-dependent manner with IC50 of 83.629 mg/mL. Novelty: This study showed that Cu-Cu2O NPs synthesized from U. wightii are a good antioxidant agent, potent antimicrobial, and cytotoxicity, hence can be used in clinical therapeutic applications.

Keywords: Urginea wightii; green synthesis; antioxidant; antimicrobial; cytotoxicity


  1. Ngoccuong H, Pansambal S, Ghotekar S, Oza R, Thithanhhai N, Nguyen VH. New frontiers in the plant extract mediated biosynthesis of copper oxide (CuO) nanoparticles and their potential applications: A review. Environmental Research. 2022;203:111858. Available from: https://doi.org/10.1016/j.envres.2021.111858
  2. Nilanjan C, Jishnu B, Pallab C, Anuron B, Sumeddha C, Kasturi R, et al. Green synthesis of copper/copper oxide nanoparticles and their applications: a review. Green Chemistry Letters and Reviews. . Available from: https://doi.org/10.1080/17518253.2022.2025916
  3. Raveesha HR, Bharath HL, Vasudha DR, Sushma BK, Pratibha S, Dhananjaya N. Antibacterial and antiproliferation activity of green synthesized nanoparticles from rhizome extract of Alpinia galangal (L.) Wild. Inorganic Chemistry Communications. 2021;132:108854. Available from: https://doi.org/10.1016/j.inoche.2021.108854
  4. Cuong HN, Pansambal S, Ghotekar S, Oza R, Hai NTT, Viet NM, et al. New frontiers in the plant extract mediated biosynthesis of copper oxide (CuO) nanoparticles and their potential applications: A review. Environmental Research. 2022;203:111858. Available from: https://doi.org/10.1016/j.envres.2021.111858
  5. Nguyen NTT, Nguyen LM, Nguyen TTT, Nguyen TT, Nguyen DTC, Tran TV. Formation, antimicrobial activity, and biomedical performance of plant-based nanoparticles: a review. Environmental Chemistry Letters. 2022;p. 1–41. Available from: https://doi.org/10.1007/s10311-022-01425-w
  6. Vasantharaj S, Sathiyavimal S, Senthilkumar P, Lewisoscar F, Pugazhendhi AFL. Biosynthesis of iron oxide nanoparticles using leaf extract of Ruellia tuberosa: Antimicrobial properties and their applications in photocatalytic degradation. Journal of Photochemistry and Photobiology B: Biology. 2019;192:74–82. Available from: https://doi.org/10.1016/j.jphotobiol.2018.12.025
  7. Raizada P, Vaid P, Saini A, Saini R. Biogenic silver, gold and copper nanoparticles- A sustainable green chemistry approach for cancer therapy. Sustainable Chemistry and Pharmacy. 2020;16:100247. Available from: https://doi:10.1016/j.scp.2020.100247
  8. Divya AJ, S. Karyomorphological and morphometric studies in Urginea wightii. International Journal of Botany studies. 2021;6(6):534–539. Available from: https://www.botanyjournals.com/archives/2021/vol6/issue6/6-6-46
  9. Raj S, Shivakameshwari. Extraction, isolation and characterization of phytoconstituents in Urginea wightii. European Journal of Medicinal Plants. 2020;p. 1–8. Available from: http://doi:10.9734/ejmp/2019/v30i430198
  10. Durak T, Depciuch J. Effect of plant sample preparation and measuring methods on ATR-FTIR spectra results. Environmental and Experimental Botany. 2020;169:103915. Available from: https://doi.org/10.1016/j.envexpbot.2019.103915
  11. Keshari AK, Srivastava R, Singh PPV, Yadav VB, Nath G. Antioxidant and antibacterial activity of silver nanoparticles synthesized by Cestrum nocturnum. Journal of Ayurveda and Integrative Medicine. 2020;11(1):37–44. Available from: https://doi.org/10.1016/j.jaim.2017.11.003
  12. Ilyasov IR, Beloborodov VL, Selivanova IA, Terekhov RP. ABTS/PP Decolorization Assay of Antioxidant Capacity Reaction Pathways. International Journal of Molecular Sciences. 2020;21(3):1131. Available from: http://doi:10.3390/ijms21031131
  13. Qamar H, Rehman S, Chauhan DK, Tiwari AK, Upmanyu V. Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from <em>Momordica charantia</em></p>. International Journal of Nanomedicine. 2020;15:2541–2553. Available from: https://doi.org/10.2147/IJN.S240232
  14. Zhang D, Ramachandran G, Mothana RA, Siddiqui NA, Ullah R, Almarfadi OM, et al. Biosynthesized silver nanoparticles using Caulerpa taxifolia against A549 lung cancer cell line through cytotoxicity effect/morphological damage. Saudi Journal of Biological Sciences. 2020;27(12):3421–3427. Available from: https://doi.org/10.1016/j.sjbs.2020.09.017
  15. Dayana KS, Jothimani R, Velladurai SC. Green synthesis of copper oxide nanoparticles from Catharanthus roseus plant leaf extracts and its investigation. Journal of Nano and Electronic Physics. 2021;1(13):1014. Available from: https://doi:10.21272/jnep.13(1).01014
  16. Jayarambabu N, Akshaykranth A, Rao TV, Rao KV, Kumar RR. Green synthesis of Cu nanoparticles using Curcuma longa extract and their application in antimicrobial activity. Materials Letters. 2020;259(15):126813. Available from: https://doi.org/10.1016/j.matlet.2019.126813
  17. Kavitha G, Vinoth KJ, Abirami N, Arulmozhi R, Siranjeevi R, Satish R. Green synthesis of Copper oxide nanoparticles decorated with grapheme oxide for anticancer activity and catalytic applications. Arabian Journal of Chemistry. 2020;13(8):6802–6814. Available from: https://doi.org/10.1016/j.arqbjc.2020.06.033


© 2022 Raveesha & Pramila L. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Published By Indian Society for Education and Environment (iSee)


Subscribe now for latest articles and news.