Indian Journal of Science and Technology

Abstract

Controlled release of typhoid antigen encapsulated potato starch microparticles minimize the disadvantages in terms of cost, multiple doses and side effects. The development of this work may enable to replace the alum to elicit sustained immune response because alum induces local granuloma and hypersensitivity reaction to some individuals. We have employed emulsion crosslinking technique by using 0.5% ml glutaraldehyde as a crosslinking agent. The particle size was analyzed as 43.34 µm; the loading capacity was about 44.76% and the loading efficiency as about 75.17%, while the morphology was unchanged in the stability studies. In vitro studies were analyzed by SEM and compatibility studies were performed by IR α F.T spectroscopic analysis. The percentage of antigenically active typhoid antigens was found to be 91.5% by ELISA after entrapment with 8% of starch and 0.5% of stabilizing agent. Immunoprecipitation assay exhibited that the amount of antigen in the sample was found to be 51µg and at the dilution of 1:50 showed agglutination factor (with the titer value of 50).
Keywords: Typhoid, potato starch, biodegradability, immunomodulatory, single therapy, antibody response.