In vitro Shoot Micro Propagation of Medicinal Applications and Ornamental Value of Cestrum nocturnum L.

Mohammad Khairul Islam Sarkar; Miskat Ara Akhter Jahan; Chapol Kumar Roy; Khaled Hossain; Shafkat Shamim Rahman; Md  Rubel Mia; Mohammad Rafiqul Islam  and Dewan Anwarul Azim

doi:10.17485/ijst/2016/v9i15/86233

Article

In vitro Shoot Micro Propagation of Medicinal Applications and Ornamental Value of Cestrum nocturnum L.

VIEWS 1305
PDF 374

Abstract
Full-Text HTML
Full-Text PDF
How to Cite

Indian Journal of Science and Technology

DOI: 10.17485/ijst/2016/v9i15/86233

Year: 2016, Volume: 9, Issue: 15, Pages: 1-7

Original Article

In vitro Shoot Micro Propagation of Medicinal Applications and Ornamental Value of Cestrum nocturnum L.

Mohammad Khairul Islam Sarkar^1*, Miskat Ara Akhter Jahan² , Chapol Kumar Roy² , Khaled Hossain³ , Shafkat Shamim Rahman⁴, Md. Rubel Mia³ , Mohammad Rafiqul Islam¹ and Dewan Anwarul Azim³

¹Independent University, Bangladesh (IUB), Dhaka, Bangladesh; [email protected], [email protected] ²Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh; [email protected], [email protected] ³Primeasia University, Dhaka, Bangladesh; [email protected], [email protected], [email protected] ⁴BRAC University, Dhaka, Bangladesh; [email protected]

*Author of Corresponding: Mohammad Khairul Islam Sarkar Independent University, Bangladesh (IUB), Dhaka, Bangladesh; [email protected]

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background/Objectives: Cestrum nocturnum L. a night Blooming jasmine belongs to Solanaceae family widely circulated all over tropical as well as subtropical areas of the World. It is mainly popular for ornamental fragrant flowering and hedge plant but also sometimes for traditional medicinal purpose. Due to strongest smelling characteristics of the plants, it is used in many industries for making Perfumes, essential Oils, Soaps, Candles, Body Oils, etc. The existence of natural plants of economic importance are threatened due to rapid urban development, including industrialization, residential development, educational, commercial etc., reduce the land for cultivation. Hence plant tissue culture protocol may be adapted for production and utilization of economically popular plants, including C. nocturnum involving limited space and short period of time. Methods: Shoot tip explants of naturally grown C. nocturnum were excised sterilized and endued on ‘Murashige and Skoog’ (MS) medium enriched changed concentration of BA, NAA, as well as GA3 singly or in combination. Excised micro shoots were examined for root development on 0.5 MS using IBA, NAA as well as IAA separately. Findings: The highest amount of multiple bud were observed in low concentration of BA (01.50 milligram × l-1), resulted no. of shoot 4.40 as well as 4.20/explant, no. of leaves 15.40 as well as 4.20/explant as well as size of different shoot 5.360 as well as 4.860 cm. The concentration of IBA and IAA were found to be best for root formation in micro shoots (13.20, 6.80 roots/micro shoots) as well as root size (8.39, 5.73 cm) individually. Application: There are many opportunity of plant tissue culture which offer marvelous chances in plant propagation, plant development as well as creation of plants with necessary agronomical features. Finally often hardening plantlets were gradually adjusted to natural condition and acclimatized with 90% success. This established protocol could help plant cell biotechnology, horticulture, medical and industrial sector of the country.

Keywords: Cestrum nocturnum, Micro Propagation, MS Salts Medium, Night Jasmine, Tissue Culture, Transplantable Plantlets